EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence


, : Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence. Veterinary Immunology and Immunopathology 53(3-4): 303-311

Luminol- and lucigenin-dependent chemiluminescence (CL) was used to compare activation of the respiratory burst of chicken peripheral blood monocytes and heterophils after stimulation with various agents. Monocytes and heterophils were obtained from the blood of three specific-pathogen-free chickens at 14 months of age and purified by a two-step discontinuous Percoll gradient. All cells responded to phorbol 12-myristate 13-acetate (PMA), zymosan A and calcium ionophore A23187 producing CL. The time course of luminol- and lucigenin-dependent CL was similar for both monocytes and heterophils after stimulation with PMA or zymosan A. Heterophils at lower cell number than monocytes responded with similar or higher peak maximum (PM) values. At the concentrations of stimuli used, the order of mean PM values was: zymosan A gt PMA gt A23187. Addition of 4 times 10-6 M N-formyl-1-L-methionyl-L-leucyl-L-phenylalanine (fMLP) showed weak but significant CL activity at 1 times 10-6 monocytes per tube with luminol and at 5 times 10-6 monocytes per tube with lucigenin. No significant response to fMLP was observed with heterophils. The results indicate that the respiratory burst of chicken monocytes and heterophils can be measured by CL.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914218

PMID: 8969050

DOI: 10.1016/S0165-2427(96)05620-6

Download PDF Full Text: Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Poon, R.Tung-Ping.; Ng, I.Oi-Lin.; Lau, C.; Yu, W-Ching.; Yang, Z-Fan.; Fan, S-Tat.; Wong, J., 2002: Tumor microvessel density as a predictor of recurrence after resection of hepatocellular carcinoma: a prospective study. This study prospectively evaluated the correlation of tumor microvessel density (MVD) with clinicopathologic features and postoperative recurrence in patients undergoing resection of hepatocellular carcinoma (HCC). Tumor MVD was assessed in 100 pa...

Wyss, A.P.; Poe, C.F., 1948: The effect of benzoic acids on spoilage organisms. Phenol coeffs. are reported for 11 benzoic acids as detd. by the U.S. F.D.A. testing method using as test organisms the food spoilage bacteria Lactobacillus lycopersici, L. mannitopoeus, L. pentoaceticus, and L. plantarum. Values for the same acid...

Roberge, F.G.; Kozhich, A.; Chan, C.C.; Martin, D.F.; Nussenblatt, R.B.; D.S.et, M.D., 1993: Inhibition of cellular transfer of experimental autoimmune uveoretinitis by Rapamycin. The crucial role of CD4(+) T cells in mediating uveitis is well recognized. One treatment strategy of non-infectious uveitis therefore seeks to inhibit T cell function. For that purpose the authors have evaluated the efficacy of Rapamycin (RAPA),...

Sugawara, K., 1980: Management of refractory diseases at the clinic level. Kangogaku Zasshi 44(1): 60-61

Edelson J.D.; Post M.; Vadas P.; Pruzanski W.; Chan S., 1991: Surfactant protein a expression in phospholipase a 2 induced lung injury. American Review of Respiratory Disease 143(4 PART 2): A727

Mantovani, B.; Scali, V., 1988: Bacillus atticus (Insecta Phasmatodea) in Sardinia (new record) and its relationship with B. rossius. The distribution in the Mediterranean Basin of 10 taxa of Bacillus is described and illustrated. The holomediterranean genus Bacillus comprises two bisexual taxa (B. rossius and B. grandii) and 8 thelytokous ones, among which the B. atticus comple...

Thorén, P., 1972: Left ventricular receptors activated by severe asphyxia and by coronary artery occlusion. Virus-specific complementary ribonucleic acid (RNA) from cells infected with Sendai virus was isolated by a procedure involving hybridization with virion RNA and isopycnic centrifugation of the RNA hybrids. The complementary RNA contained adenylat...

Rokhade, A., P.; Shelke, N., B.; Patil, S., A.; Aminabhavi, T., M., 2007: Novel interpenetrating polymer network microspheres of chitosan and methylcellulose for controlled release of theophylline. Interpenetrating polymer network (IPN) microspheres of chitosan (CS) and methylcellulose (MC) were prepared by emulsion-crosslinking in the presence of glutaraldehyde (GA) as a crosslinker. Theophylline (THP), an antiasthmatic drug was encapsulat...

Radzyminska, M.; Smoczynski, S.S., 2006: Stall and pasture feeding as a factor potentially affecting the content of detrimental chemical compounds in selected dairy products. The study was aimed at determining the impact of the milk production period and selected dairy products on the level of external chemical compound residues in milk. For this reason the experimental material was sampled in the summer and in winter...

Narayanan, K., 1985: Susceptibility of Spilosoma obliqua Walker to Nosema sp. The pathogenicity and susceptibility of all 6 instars of the arctiid pest Spilosoma obliqua to the pathogen Nosema sp., isolated from larvae of S. obliqua for the first time in India, was examined. The sympthoms of Nosema-infected S. obliqua gener...