+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence

, : Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence. Veterinary Immunology and Immunopathology 53(3-4): 303-311

Luminol- and lucigenin-dependent chemiluminescence (CL) was used to compare activation of the respiratory burst of chicken peripheral blood monocytes and heterophils after stimulation with various agents. Monocytes and heterophils were obtained from the blood of three specific-pathogen-free chickens at 14 months of age and purified by a two-step discontinuous Percoll gradient. All cells responded to phorbol 12-myristate 13-acetate (PMA), zymosan A and calcium ionophore A23187 producing CL. The time course of luminol- and lucigenin-dependent CL was similar for both monocytes and heterophils after stimulation with PMA or zymosan A. Heterophils at lower cell number than monocytes responded with similar or higher peak maximum (PM) values. At the concentrations of stimuli used, the order of mean PM values was: zymosan A gt PMA gt A23187. Addition of 4 times 10-6 M N-formyl-1-L-methionyl-L-leucyl-L-phenylalanine (fMLP) showed weak but significant CL activity at 1 times 10-6 monocytes per tube with luminol and at 5 times 10-6 monocytes per tube with lucigenin. No significant response to fMLP was observed with heterophils. The results indicate that the respiratory burst of chicken monocytes and heterophils can be measured by CL.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002914218

PMID: 8969050

DOI: 10.1016/S0165-2427(96)05620-6

PDF Full Text: Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Glaesner, N.; Kjaergaard, C.; Rubaek, G.H.; Magid, J., 2011: Interactions between soil texture and placement of dairy slurry application: I. Flow characteristics and leaching of nonreactive components. Land application of manure can exacerbate nutrient and contaminant transfers to the aquatic environment. This study examined the effect of injecting a dairy cattle (Bostaurus L.) manure slurry on mobilization and leaching of dissolved, nonreactive...

Hess, R.F.; Li, X.; Mansouri, B.; Thompson, B.; Hansen, B.C., 2010: Selectivity as well as sensitivity loss characterizes the cortical spatial frequency deficit in amblyopia. The processing deficit in amblyopia is not restricted to just high spatial frequencies but also involves low-medium spatial frequency processing, for suprathreshold stimuli with a broad orientational bandwidth. This is the case in all three forms...

Wirth Richard; Marks Michael; Markl Gregor, 1999: Exsolution lamellae of augitic clinopyroxene and magnetite in olivine Fa60-90 from the augite syenite unit of the Ilimaussaq Intrusion, South Greenland. Eos, Transactions, American Geophysical Union 80(46, Suppl

Devereux; Hines; Stahl, 1996: S Cycling: Characterization of Natural Communities of Sulfate-Reducing Bacteria by 16S rRNA Sequence Comparisons. Past studies of microbial communities responsible for geochemical transformations have been limited by an inability to representatively cultivate, and then identify, the constituent members. Ribosomal RNA sequences, particularly 16S-like rRNAs, pr...

Sheu, K.F.R.; Frey, P.A., 1978: Udp glucose pyro phosphorylase ec stereochemical course of the reaction of glucose 1 phosphate with uridine 5 1 thio tri phosphate. Of the 2 diastereoisomers of uridine -5'[1-thiotriphosphate] differing in configuration at P.alpha., [bakers yeast] UDP-glucose pyrophosphorylase (EC catalyzes the reaction of the one designated isomer B with glucose-1-phosphate to p...

Alwar R.P.A., 1985: Nutrients extracted by crop a basis for fertilizer recommendation. Journal of Coffee Research 15(3-4): 15-16

Solveborn, S. A., 1997: Radical epicondylalgia ('tennis elbow'): Treatment with stretching or forearm band. A prospective study with long-term follow-up including range-of-motion measurements. Stretching or upper forearm bands were used in the treatment of radial epicondylalgia ('tennis elbow') in a prospective study of 185 patients. The two consecutive series did not differ with respect to, for example, age (median 43 years),...

King, J.R.; Withler, R.E., 2005: Male nest site fidelity and female serial polyandry in lingcod (Ophiodon elongatus, Hexagrammidae). Nest site fidelity and serial polyandry were examined in lingcod, Ophiodon elongatus, a teleost fish in which the nest-guarding male parent invests more heavily in parental care than the elusive female parent. Lingcod parental and progeny genotype...

ABEL, T.M., 1953: Figure drawings and facial disfigurement. American Journal of Orthopsychiatry 23(2): 253-264

Van Den Berg, J.; Rebe, M.; D.B.uyn, J.; Van Hamburg, H., 2001: Developing habitat management systems for gramineous stemborers in South Africa. The stemborers Busseola fusca (Lepidoptera: Noctuidae) and Chilo partellus (Lepidoptera: Crambidae) are important pests of maize and sorghum in South Africa. A habitat management system for these pests is being developed in the Northern Province o...