+ Translate

Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence

, : Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence. Veterinary Immunology and Immunopathology 53(3-4): 303-311

Luminol- and lucigenin-dependent chemiluminescence (CL) was used to compare activation of the respiratory burst of chicken peripheral blood monocytes and heterophils after stimulation with various agents. Monocytes and heterophils were obtained from the blood of three specific-pathogen-free chickens at 14 months of age and purified by a two-step discontinuous Percoll gradient. All cells responded to phorbol 12-myristate 13-acetate (PMA), zymosan A and calcium ionophore A23187 producing CL. The time course of luminol- and lucigenin-dependent CL was similar for both monocytes and heterophils after stimulation with PMA or zymosan A. Heterophils at lower cell number than monocytes responded with similar or higher peak maximum (PM) values. At the concentrations of stimuli used, the order of mean PM values was: zymosan A gt PMA gt A23187. Addition of 4 times 10-6 M N-formyl-1-L-methionyl-L-leucyl-L-phenylalanine (fMLP) showed weak but significant CL activity at 1 times 10-6 monocytes per tube with luminol and at 5 times 10-6 monocytes per tube with lucigenin. No significant response to fMLP was observed with heterophils. The results indicate that the respiratory burst of chicken monocytes and heterophils can be measured by CL.

Accession: 002914218

PMID: 8969050

DOI: 10.1016/S0165-2427(96)05620-6

Download PDF Full Text: Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Desmidt, M.N.rom, A.; Haesebrouck, F.D.catelle, R.Y.ebaert, M., 1996: Oxygenation activity of chicken blood phagocytes as measureed by luminol-and lucigenin-dependent chemiluminescence. Veterinary immunology and immunopathology 53(3-4): 303-311

G.V.M.karskaya; S.V.T.rskikh; E.G.T.ritsyna, 2011: Luminol- and lucigenin-dependent chemiluminescence of chicken whole-blood cells during postnatal ontogeny. It is shown by the luminol- and lucigenin-dependent chemiluminescence method that oxygen metabolism in chicken whole-blood cells is characterized by the prevalence of the generation of primary reactive oxygen species (superoxide anion) both in a s...

Zhang, R.Q., 1989: Effect of cigarette-smoke tar on oxygenation activity of granulocytes detected by luminol- and lucigenin-enhanced chemiluminescence response of rabbit whole blood. Both luminol- and lucigenin-dependent rabbit whole blood chemiluminescence showed a concentration-dependent inhibition response to cigarette-smoke tar of 0.26 to 2.6 mg/ml. Tar showed no cytotoxicity to granulocytes by Evans blue exclusion. It is...

Pavelkova, M.; Kubala, L., 2004: Luminol-, isoluminol- and lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators. Luminol-, isoluminol- or lucigenin-enhanced chemiluminescence (CL) was used to measure the production of reactive oxygen species by rat blood leukocytes. Opsonized zymosan (OZ), phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 (Ca-I...

Jae Hyuck Jung; S.R.un Kwon; Eun Hye Lee; Sung M.K.m; Hyun; Joon K.C.ung; Kim,, 2003: Comparison between lucigenin- and luminol-dependent chemiluminescence responses of rockfish (Sebastes schlegeli) head kidney phagocytes. Journal of Fisheries Science and Technology. December; 64: 209-212

Falck P., 1986: Lucigenin and luminol reinforced chemiluminescence of stimulated human phagocytes. Wissenschaftliche Zeitschrift Der Humboldt Universitaet Zu Berlin Mathematisch Naturwissenschaftliche Reihe: 49-51

Shkapova, E.A.; Kurtasova, L.M.; Savchenko, A.A., 2011: Lucigenin- and luminol-dependent chemiluminescence of blood neutrophils in patients with renal cancer. High basal production of primary active oxygen forms was detected in the peripheral blood neutrophils of patients with renal cell cancer. In vitro stimulation of neutrophils led to more rapid release of superoxide radicals into extracellular space...

Greger, E.A.; Drum, A.S.; Elston, R.A., 1995: Measurement of oxidative activity in hemocytes of the pacific razor clam, Siliqua patula, and the oyster, Crassostrea gigas, using lucigenin- and luminol-dependent chemiluminescence. In a manner resembling the respiratory burst of activated mammalian polymorphonuclear leukocytes, hemocytes of the Pacific razor clam, Siliqua patula, produced reactive oxygen intermediates during in vitro phagocytosis of zymosan particles. The ac...

Klimshina O.A.; Erin A.N., 1991: Luminol and lucigenin dependent chemiluminescence under 6 hydroxydopamine autooxidation. It was shown by the methods of lumonol- and lucigenin-dependent chemiluminescence that 6-hydroxydopamine 6-ONDA autooxidation is accompanied by generation of superoxide anionradical which can further react with next 6-ONDA molecule. This fact is b...

Abdullah, A.S.; Jawad, A.M.; Al-Hashimi, A.H., 2001: In vitro effect of different non-steroidal anti-inflammatory drugs on human polymorphonuclear leukocyte activity measured by luminol-dependent chemiluminescence of the whole blood. Objectives: To define the well-known variability in the effects of non-steroidal anti-inflammatory drugs and to search for predictors of such variability using an in vitro model. Methods: Polymorphonuclear leukocyte activity was measured by lumino...