EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy


, : Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy. Animal Reproduction Science 41(1): 61-70

Oxytocin (OT) receptors in the porcine endometrium were investigated at four stages of the estrous cycle (Days (D) 0, 5, 10 and 15, n = 3), and at two stages of early pregnancy (D5 and D15 after mating, n = 3) by a radioreceptor assay using 125I-labeled OT antagonist (d(CH-2)-5,Tyr(Me)-2,Thr-4,Tyr-NH-2-9)-vasotocin. Binding specificity was demonstrated by displacement with four peptides related to oxytocin ((Arg-7)-vasopressin, (Thr-4,Gly-7)-OT, OVT, OT) and two peptides unrelated to oxytocin (luteinizing hormone-releasing hormone, (Ile-3)-pressinoic acid (tocinoic acid)). The dissociation constant (K-d) of endometrial OT receptors on D0 (0.59 +- 0.10 nM) was similar to those on D10 and D15 (D10, 0.75 +- 0.21; D15, 0.60 +- 0.14 nM; mean +- SEM). In the early luteal stage (D5), K-d (2.41 +- 0.24 nM) was higher than on D0, D10 and D15 (P lt 0.01). In early pregnancy, K-d values were 3.25 +- 0.29 nM on D5 and 2.44 +- 0.44 nM on D15. Binding site concentration (B-max) on D0 (910.0 +- 25.1 fmol mg-1 protein) was significantly higher than on D5 and D10 (D5, 322.5 +- 71.7; D10, 147.5 +- 25.8 fmol mg-1 protein; P lt 0.01) of the estrous cycle and D5 and D15 (D5, 302.5 +- 82.6; D15, 315.0 +- 20.1 fmol mg-1 protein; P lt 0.01) of early pregnancy. In the two stages of early pregnancy, B-max values were constant and similar to that on D5 of the early luteal stage. Our results reveal the existence of specific OT binding sites in the porcine endometrium during the estrous cycle and early pregnancy. Furthermore, the fluctuation in the binding of OT to the endometrium during the different stages of the estrous cycle suggests that OT plays an important role in regulating the estrous cycle of the pig as seen in other animals.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914236

DOI: 10.1016/0378-4320(95)01439-x

PDF Full Text: Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Tresilian, James R., 1998: Attention in action or obstruction of movement? A kinematic analysis of avoidance behavior in prehension. Obstacle avoidance strategies are of two basic but interrelated types: moving around an obstacle to that body parts do not come too close, and slowing down. In reaching-to-grasp, avoidance may involve the transport component, the grasp formation c...

Al-Sarheed, M.Abdulla., 2006: Evaluation of shear bond strength and SEM observation of all-in-one self-etching primer used for bonding of fissure sealants. To evaluate and compare enamel shear bond strength of an all-in-one self-etching primer (Prompt L-Pop) to regular acid etch material when bonded to two-fissure sealant systems (Concise and Dyract Seal). Forty newly extracted non-carious first perm...

Aoyagi, Y., 1935: Nachweis des Impeding in den Tuberkulinpraparaten, zugleich die Kritik uber ihre Herstellungsmethode sowie die staatliche Kontrollierung der Wertigkeit der Praparate. In comparative test of Koch's O.T. and koktigen by phagocytosis, Phagocytic power was improved by 20 min. boiling, of all samples of O.T. tested except that of Hokken, proving the presence of impedin in these preparations. No impedin was foun...

Staalduine, D. van, 1976: Some topical points concerning the use of herbicides. Data are presented on the relative cumulative yields of young apple trees receiving a range of herbicides for 8 years, or with a sward mown as necessary; the yields of apple trees receiving herbicides for 8 years, compared with no herbicides, limi...

Eilam, T.; Anikster, Y.; Millet, E.; Manisterski, J.; Sagi-Assif, O.; Feldman, M., 2007: Genome size and genome evolution in diploid Triticeae species. One of the intriguing issues concerning the dynamics of plant genomes is the occurrence of intraspecific variation in nuclear DNA amount. The aim of this work was to assess the ranges of intraspecific, interspecific, and intergeneric variation in...

Meyenburg, M.; Busch, W., 1976: External cephalic version using tocalytica. 1. This report relates our experiences with 63 external versions of random breech or transverse presentations in cranial presentation between the 35th and 40th week of pregnancy p.m. using tocolytica. Only 22 of the 63 versions were successful whe...

Haugli, F.B.; Dove, W.F., 1972: Mutagenesis and mutant selection in Physarum polycephalum. Molecular & General Genetics 118(2): 109-124

Nikolaeva L.F.; Aleshin O.I.; Pavlov N.A.; Pomerantsev E.V.; Masenko V.P.; Baranov A.V.; Kupriyanenko T.I., 1988: The prostacyclin thromboxane a 2 system during induced myocardial ischemia in coronary patients. Sovetskaya Meditsina (4): 3-6

Nikinmaa, M.; Salama, A.; Bogdanova, A.; Virkki, L.V., 2001: Beta-adrenergic stimulation of volume-sensitive chloride transport in lamprey erythrocytes. We measured the effects of a beta-adrenergic agonist, isoproterenol, on chloride transport and volume regulation of lamprey (Lampetra fluviatilis) erythrocytes in isotonic (288 mosm L(-1)) and hypotonic (192 mosm L(-1)) medium. Isoproterenol at a...

Gradnik, B., 1969: Effect of phosphocreatine on digoxin poisoning in vivo. Bollettino Della Societa Italiana di Biologia Sperimentale 45(9): 619-622