EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy


, : Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy. Animal Reproduction Science 41(1): 61-70

Oxytocin (OT) receptors in the porcine endometrium were investigated at four stages of the estrous cycle (Days (D) 0, 5, 10 and 15, n = 3), and at two stages of early pregnancy (D5 and D15 after mating, n = 3) by a radioreceptor assay using 125I-labeled OT antagonist (d(CH-2)-5,Tyr(Me)-2,Thr-4,Tyr-NH-2-9)-vasotocin. Binding specificity was demonstrated by displacement with four peptides related to oxytocin ((Arg-7)-vasopressin, (Thr-4,Gly-7)-OT, OVT, OT) and two peptides unrelated to oxytocin (luteinizing hormone-releasing hormone, (Ile-3)-pressinoic acid (tocinoic acid)). The dissociation constant (K-d) of endometrial OT receptors on D0 (0.59 +- 0.10 nM) was similar to those on D10 and D15 (D10, 0.75 +- 0.21; D15, 0.60 +- 0.14 nM; mean +- SEM). In the early luteal stage (D5), K-d (2.41 +- 0.24 nM) was higher than on D0, D10 and D15 (P lt 0.01). In early pregnancy, K-d values were 3.25 +- 0.29 nM on D5 and 2.44 +- 0.44 nM on D15. Binding site concentration (B-max) on D0 (910.0 +- 25.1 fmol mg-1 protein) was significantly higher than on D5 and D10 (D5, 322.5 +- 71.7; D10, 147.5 +- 25.8 fmol mg-1 protein; P lt 0.01) of the estrous cycle and D5 and D15 (D5, 302.5 +- 82.6; D15, 315.0 +- 20.1 fmol mg-1 protein; P lt 0.01) of early pregnancy. In the two stages of early pregnancy, B-max values were constant and similar to that on D5 of the early luteal stage. Our results reveal the existence of specific OT binding sites in the porcine endometrium during the estrous cycle and early pregnancy. Furthermore, the fluctuation in the binding of OT to the endometrium during the different stages of the estrous cycle suggests that OT plays an important role in regulating the estrous cycle of the pig as seen in other animals.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914236

DOI: 10.1016/0378-4320(95)01439-x

Download PDF Full Text: Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Aro, E.; Khatri, R.; Gerard-O'Riley, R.; Mangiavini, L.; Myllyharju, J.; Schipani, E., 2013: Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes. Hypoxia-inducible factors (HIFs) are the master regulators of hypoxia-responsive genes. They play a critical role in the survival, development, and differentiation of chondrocytes in the avascular hypoxic fetal growth plate, which is rich in extra...

LAUTER, H., 1964: Pathological 3-Phase Rhythm In Diencephalic Diseases. Archiv für Psychiatrie und Nervenkrankheiten 205: 149-164

Braungart-Rieker, J.M.; Hill-Soderlund, A.L.; Karrass, J., 2010: Fear and anger reactivity trajectories from 4 to 16 months: the roles of temperament, regulation, and maternal sensitivity. Two goals guided this study: (a) describe changes in infant fear and anger reactivity from 4 to 16 months and (b) examine the degree to which infant temperament, attentional regulation, and maternal sensitivity predict reactivity trajectories. Par...

Böhme, H.R.; Barthel, U.G., 1978: Influencing the automatized chelesterol determination using the Liebermann-Burchard technic through selected drugs in vitro. The ascertained influences of this cholesterol determination in vitro mentioned in the survey literature for therapeutically relevant doses are astonishingly small. In the present paper the following drugs were tested: Dextran, vitamin C, procain,...

Buser, H. R., 1975: Poly chlorinated di benzo p dioxins separation and identification of isomers by gas chromatography mass spectrometry. Attempts were made to synthesize all polychlorinated dibenzo-p-dioxin [PCDD] isomers containing 6-8 Cl atoms by micro-scale pyrolysis of different polychlorophenates. Eight of the 10 possible hexachlorodibenzo-p-dioxins, the 2 hepta- and the octac...

BERG, G., 1951: The reaction of the blood brain barrier in anaphylactic shock. Zeitschrift für die Gesamte Experimentelle Medizin 118(2): 123-130

Hance, R.J.; Holly, K., 1990: The properties of herbicides. Information is given on the bases of herbicide selectivity, method of herbicide use and mode of action, followed by brief descriptions of the properties and main uses of the most important herbicides available commercially in the UK and elsewhere....

Fujita, K.; Yoshioka, H., 1977: Relevance of concentration of pathogenic bacteria in cerebrospinal fluid to antibiotic therapy. Journal of Pediatrics 90(2): 328-329

Baron, M.; Hudson, M.; Steele, R.; Pope, J.; Markland, J.; Robinson, D.; Khalidi, N.; Jones, N.; Masetto, A.; Kaminska, E.; Docherty, P.; Mathieu, J-Pierre.; Sutton, E.; Abu-Hakima, M.; LeClercq, S.; Smith, D.; Ligier, S.; Mittoo, S.; Fritzler, M., 2010: Is serum albumin a marker of malnutrition in chronic disease? The scleroderma paradigm. Malnutrition is common in many chronic diseases, but physicians may rely on a low albumin value before deciding that malnutrition is present. To determine the relationship between serum albumin and malnutrition in systemic sclerosis (SSc) as a par...

Whitney John W.; Trousdale William, 1984: Man versus sand in Southwest Afghanistan. Abstracts with Programs - Geological Society of America 16(6): 693