EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR amplification of species-specific DNA sequences can distinguish among Phytophthora species


, : PCR amplification of species-specific DNA sequences can distinguish among Phytophthora species. Applied and Environmental Microbiology 60(7): 2616-2621

We used PCR to differentiate species in the genus Phytophthora, which contains a group of devastating plant pathogenic fungi. We focused on Phytophthora parasitica, a species that can infect solanaceous plants such as tomato, and on Phytophthora citrophthora, which is primarily a citrus pathogen. Oligonucleotide primers were derived from sequences of a 1,300-bp P. parasitica-specific DNA segment and of an 800-bp P. citrophthora-specific segment. Under optimal conditions, the primers developed for P. parasitica specifically amplified a 1,000-bp sequence of DNA from isolates of P. parasitica. Primers for P. citrophthora similarly and specifically amplified a 650-bp sequence of DNA from isolates of P. citrophthora. Detectable amplification of these specific DNA sequences required picogram quantities of chromosomal DNA. Neither pair of primers amplified these sequences with DNAs from other species of Phytophthora or from the related genus Pythium. DNAs from P. parasitica and P. citrophthora growing in infected tomato stem tissue were amplified as distinctly as DNAs from axenic cultures of each fungal species. This is the first report on PCR-driven amplification with Phytophthora species-specific primers.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914390

PMID: 8074533

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Luettig G., 1971: The german turkish lignite exploration in turkey of the years 1965 to 1969 plan of operation and scientific mainly stratigraphic results. Newsletters On Stratigraphy: 11-18

Ekman, E.; Börjeson, H.; Johansson, N., 1999: Flavobacterium psychrophilum in Baltic salmon Salmo salar brood fish and their offspring. Baltic salmon brood fish were investigated for the presence of Flavobacterium psychrophilum in the kidney, spleen, brain and sexual products (ovarian fluid, unfertilized eggs and milt). Samples for bacteriology were taken at capture, when the fish...

Ozawa, T.; Takesige, T.; Kudo, Y.; Unno, H., 1955: A study on the electrocardiogram of the cray-fish, Cambarus clarkii. Zoological Magazine Tokyo, 64: 178-181

Stanley, E., 1976: Yellow warbler and a loggerhead shrike at Theodore Wirth park. Loon, 484: 188-189

Zhou-Wei; Zhou-Zheng-Bo, 2002: Domestic and imported anethol trithione in treatment of xerostomia after radiation therapy. AIM: To determine the effect of domestic and imported anethol trithione (ANTT) in treatment of xerostomia after radiation therapy. METHODS: Forty patients with xerostomia after head and neck radiation were randomly divided into two groups, twenty...

Anonymous, 1985: Questions and answers about breast lumps and breast cancer. This brochure answer some of the most common questions about benign breast lumps and breast cancer. It discusses diagnosis, treatment and prevention. In addition, it contains illustrations of self-breast examinations. A comprehensive list of Cance...

Hall Wallace Michelle K.; McAuliffe Carla M., 2002: Design, implementation, and evaluation of GIS-based learning materials in an introductory geoscience course. Learning with Geographic Information Systems (GIS) rather than about GIS has great potential for improving students' skills in problem solving, analysis, and spatial visualization. However, little is known about how well GIS-based learning li...

Yamamoto N., 1989: Effect of dmso on cytosolic ionized calcium concentration and cytoskeletal organization of hepatocytes in a primary culture. The addition of dimethyl sulfoxide (DMSO) to a chemically defined, serum free medium prolonged hepatocytes survival in primary culture. DMSO exposure had a remarkable effect on morphological change and F-actin filaments distribution of hepatocytes...

Cyunel, E; Ekiert, H., 1983: Tissue culture of Scrophularia scopoli Hoppe and investigating its metabolites. I. Tissue culture. Herba Polonica9(1): 39-43

Hager, E.R.; Candelaria, M.; Latta, L.W.; Hurley, K.M.; Wang, Y.; Caulfield, L.E.; Black, M.M., 2012: Maternal perceptions of toddler body size: accuracy and satisfaction differ by toddler weight status. To examine (1) accuracy of maternal perceptions of toddler body size; factors associated with accuracy of toddler body size; and how maternal satisfaction relates to accuracy/toddler body size. Cross-sectional. Low-income community sample from sub...