+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR assay for detection of the phytoplasma associated with maize bushy stunt disease

, : PCR assay for detection of the phytoplasma associated with maize bushy stunt disease. Plant Disease 80(3): 263-269

DNA amplification by polymerase chain reaction (PCR) was used to detect the phytoplasma associated with maize bushy stunt (MBS) disease. A pair of oligonucleotide primers was synthesized according to partial sequences of a cloned 1-kb fragment of genomic DNA of the MBS phytoplasma (Texas isolate) maintained in sweet corn. PCR performed for 30 cycles with primer annealing at 61 degree C amplified a DNA product of about 740-bp in reaction mixtures containing template DNA derived from symptomatic corn singly infected with MBS phytoplasma isolates from either Texas, Florida, Costa Rica, or Mexico. No comparable product was amplified from DNAs of healthy corn, plants affected by various other phytoplasmal diseases, or from Spiroplasma kunkelii. Forty cycles of PCR enabled detection of a Florida isolate of the MBS phytoplasma in all leaf and stalk samples tested from presymptomatic plants 12 days after plants were fed upon by inoculative vector Dalbulus maidis leafhoppers and in the majority of nonvector Peregrinus maidis planthoppers after 1 to 5 days of exposure to symptomatic plants.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002914405

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Cathébras, P.; Vautrin, R.; Boubakri, C.; Tostain, J.; Charmion, S., 2000: Four well-wrapped kidneys. La Revue de Medecine Interne 21(10): 901-902

Oda Hirokuni; Suzuki Hisashi, 2000: Paleomagnetism of Triassic and Jurassic red bed chert of the Inuyama area, central Japan. Journal of Geophysical Research, B, Solid Earth and Planets 105(11): 25,743-25,767

Pupulin, Franco, 2000: New species of Costa Rican Orchidaceae. Six new orchid species from Costa Rica are described and illustrated: Crossoglossa aurantilineata, Chondrorhyncha lankesteriana, Kefersteinia orbicularis, Ornithocephalus castelfrancoi, Sigmatostalix pseudounguiculata, and Trisetella lasiochila.

Burgoyne L.A.; Drinkwater R.; Skinner J.D., 1987: Levels of chromatin structure. Kon, O L , Et Al (Ed ) Icsu (International Council Of Scientific Unions) Short Reports, Vol 6 Contemporary Themes in Biochemistry; 4th Federation Of Asian Oceanian Biochemists Congress, Singapore, November 30-December 5, 1986 Xxxvii+715p Cambridge University Press: Cambridge, England, Uk; New York, New York, Usa Illus 150-151, 1986

de Carvalho, C.A., 1966: Considerations on the arterial architectonic of the area of the esophagogastric transition and its function interpretation. Revista do Hospital das Clinicas 21(3): 113-128

de la Torre, B.; Norén, S.; Hedman, M.; Ritzén, M.; Diczfalusy, E., 1982: Intratesticular and plasma steroid profiles in fertile and infertile men. In 24 apparently healthy men who requested sterilization by vasectomy, we have measured the intratesticular and peripheral plasma levels of 9 unconjugated and 5 sulphoconjugated steroids. These steroid levels were then compared with those found in...

Cheong, M-Leng.; Wang, L-Jie.; Chuang, P-Yun.; Chang, C-Wen.; Lee, Y-Shien.; Lo, H-Fan.; Tsai, M-Song.; Chen, H., 2016: A Positive Feedback Loop between Glial Cells Missing 1 and Human Chorionic Gonadotropin (hCG) Regulates Placental hCGβ Expression and Cell Differentiation. Human chorionic gonadotropin (hCG) is composed of a common α subunit and a placenta-specific β subunit. Importantly, hCG is highly expressed in the differentiated and multinucleated syncytiotrophoblast, which is formed via trophoblast cell fusio...

Gorbunov G.I.; Korchagin A.U.; Mednikov A.I., 1989: Mineral potential of the Pechenga picrites. Doklady Akademii Nauk SSSR 304(5): 1205-1208

Arakawa, H.; Tojo, H.; Sasao, H.; Kawano, Y.; Itami, K., 2014: A new method for determining the plasma electron density using optical frequency comb interferometer. A new method of plasma electron density measurement using interferometric phases (fractional fringes) of an optical frequency comb interferometer is proposed. Using the characteristics of the optical frequency comb laser, high density measurement...

Meyer F.P., 1992: Problems of chemotherapy in aquaculture regulatory aspects an overview. Michel, C And D J Alderman (Ed ) Chemotherapy in Aquaculture: From Theory to Reality; Conference on Problems Of Chemotherapy in Aquaculture: Theory to Reality, Paris, France, March 12-15, 1991 Xiii+567p Office International Des Epizooties: Paris, France Illus Paper 80-87