EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR confirmation of infection with Cyclospora cayetanensis


Emerging Infectious Diseases 2(4): 357-359
PCR confirmation of infection with Cyclospora cayetanensis
A total of 42 stool samples were tested for the presence of Cyclospora cayetanensis using a nested polymerase chain reaction (PCR). Light microscopy confirmed that 26 of the 42 cases were positive for Cyclospora, and a Cyclospora specific DNA fragment was amplified in 16 (62%) of these positive cases. The 10 specimens negative by PCR but positive by microscopy contained low numbers of oocysts; none of the 16 specimens which were negative by microscopy gave a positive result with PCR.


Accession: 002914408

PMID: 8969255

DOI: 10.3201/eid0204.960415



Related references

PCR-restriction fragment length polymorphism method for detection of Cyclospora cayetanensis in environmental waters without microscopic confirmation. Applied and Environmental Microbiology 69(8): 4662-4669, 2003

A case with Cyclospora cayetanensis infection. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 18(5): 319-319, 2003

A 'new' parasite: human infection with Cyclospora cayetanensis. Transactions of the Royal Society of Tropical Medicine and Hygiene 88(4): 369-371, 1994

Cyclospora cayetanensis infection in Lagos, Nigeria. Clinical Microbiology and Infection 9(7): 731-733, 2003

Cyclospora cayetanensis infection. Laboratory diagnosis. Revista Latinoamericana de Microbiologia 42(1): 45-52, 2000

A 'new' parasite: human infection with Cyclospora cayetanensis. Transactions of the Royal Society of Tropical Medicine and Hygiene 88(4): 369-371, 1994

Cyclospora cayetanensis infection in Lagos, Nigeria. Clinical Microbiology and Infection 9(7): 731-733, 2003

Co-infection with Cyclospora cayetanensis and Salmonella typhi in a patient with HIV infection and chronic diarrhoea. Pathogens and Global Health 107(1): 38-39, 2013

Emerging pathogen Cyclospora cayetanensis infection in Nepal. Southeast Asian Journal of Tropical Medicine and Public Health 32 Suppl 2: 143-150, 2002

Epidemiology of Cyclospora cayetanensis infection in San Carlos Island, Venezuela: strong association between socio-economic status and infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 101(10): 1018-1024, 2007