EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR for the detection of small ruminant lentiviruses


, : PCR for the detection of small ruminant lentiviruses. Schweizer Archiv fuer Tierheilkunde 138(2): 93-98

The polymerase chain reaction has all attributes of a promising diagnostic technique. It is rapid, simple to perform and extremely sensitive. In a PCR developed for the detection of small ruminant lentiviruses (SRLV), we found under ideal conditions a detection sensitivity up to less than 10 template DNA copies. The diagnostic application of PCR was not fully satisfying, even when the technique was refined by the use of a panel of suitable primer pairs. The reliability of PCR in blood and milk samples was much lower than that of antibody detection using ELISA. Interestingly, a positive PCR result was also recorded in 50% of the samples of seronegative animals. Seronegative lentivirus carriers due to delayed seroconversion have been described previously (Rimstad et al., 1993). Due to sporadic occurrence of false positive reactions in spite of contamination control, this result must be interpreted with caution unless the specificity of the fragments can be confirmed by sequencing. Using published sequences of SRLV, we show that sequencing of PCR products followed by phylogenetic analysis should allow to study molecular epidemiology of field strains.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914426

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Castro, A.; Delgado, JA., 1998: Notes on the presence of Hydrochus grandicollis Kiesenwetter, 1870 in the Iberian Peninsula Coleoptera, Hydrochidae Notas sobre la presencia de Hydrochus grandicollis Kiesenwetter, 1870 en la Peninsula Iberica Coleoptera, Hydrochidae. The distribution of Hydrochus grandicollis Kiesenwetter, 1870 in the south of the Iberian Peninsula is studied. Externally H. grandicollis can be easily recognized. The male genitalia of this species, very similar to that of H. nitidicollis Mulsan...

Lu, B-Sui.; Podgornik, R., 2015: Effective interactions between fluid membranes. A self-consistent theory is proposed for the general problem of interacting undulating fluid membranes subject to the constraint that they do not interpenetrate. We implement the steric constraint via an exact functional integral representation an...

Cueva, M.; Hicks, T.; Kuhnley, R.; Cueva, K., 2013: A Wellness Course for Community Health Workers in Alaska: "wellness lives in the heart of the community". To develop, implement, and evaluate a culturally respectful Wellness Course with and for Alaska's village-based Community Health Workers (CHWs) to support community health promotion and disease prevention. This article describes Wellness Cour...

Arevshatyan A.A., 1974: Effect of aeration and iron concentration on porphyrin synthesis by candida guilliermondii. Mikrobiologiya: 1041-1046

Dai, Q.; Coronel, V.P.; Vergara, B.S.; Barnes, P.W.; Quintos, A.T., 1992: Ultraviolet-B radiation effects on growth and physiology of four rice cultivars. Enhanced ultraviolet-B (UV-B, 280-320 nm) radiation, such as could be caused by stratospheric O-3 depletion, has been demonstrated to profoundly affect plants. This study was conducted to determine the effects of UV-B on four high-yielding, lowlan...

Royle, T.J.; Ferguson, H.J.M.; Mak, T.W.C.; Simpson, J.A.; Thumbe, V.; Bhalerao, S., 2015: Same-day assessment and management of urgent (2-week wait) colorectal referrals: an analysis of the outcome of 1606 patients attending an endoscopy unit-based colorectal clinic. The Rapid Access Diagnosis and Remedy (RADAR) clinic combines 2-week wait (TWW) specialist consultation with 'straight-to-test' flexible sigmoidoscopy (FS) for left-sided 'red-flag' TWW criteria (excluding right-sided mass or i...

Butera, E., 1990: Nursing in the next decade. Nephrology News & Issues 4(1): 20, 25-20, 25

Ruan, C.; Xiao, X-hua.; Li, G-ke., 2015: Microwave-assisted extraction coupled with countercurrent chromatography for the rapid preparation of flavonoids from Scutellaria barbata D. Don. As a famous Chinese herb having good inhibitory effects on numerous human cancers both in vitro and in vivo, Scutellaria barbata D. Don attracts extensive attention worldwide. In this work, four flavonoids named scutellarin, baicalin, luteolin, an...

Cobbaert, C.; Kent, P.P.; Van D.W.rf, F.; Simoons, M.L.; Lindemans, J.L., 1993: Extra myoglobin peaks corroborate the diagnosis of stuttering infarction. Circulation 88(4 PART 2): I490

Troitsky, S. K., 1959: Leuciscus borysthenicus in the Kuban basin. Ichthyological Problems Moscow, 13: 51-54