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PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin


, : PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiology Letters 150(2): 209-217

Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptospordium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.

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Accession: 002914446

DOI: 10.1111/j.1574-6968.1997.tb10372.x

PDF Full Text: PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin


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Henry, L.; Mugica, J.; Attuel, P.; Rollet, M.; Podeur, H., 1983: Why implant a cardiac pacemaker? Review of physiology and electrocardiography, diagnosis and indications. Soins. Chirurgie: 3-8

Bohus B.; Nyakas C.; D.K.oet E.R., 1978: Hippocampal function and cortico sterone receptors implication in behavioral processes. Journal of Steroid Biochemistry 9(9): 882

Halloran, M.E.; Bundy, D.A.P.; Pollitt, E., 1989: Infectious disease and the Unesco Basic Education initiative. With reference to the identification of Trichuris trichiura and Ascaris lumbricoides as targets for immediate intervention initiatives by UNESCO, this article discusses the prevalence and intensity of these infections in school children, the evide...

Anonymous, 1930: Codling Moth Control. This leaflet contains practical advice on the control of the codling moth based on extensive experiments . Further tests are being made to determine what interval must elapse between the use of oil and lime-sulphur sprays in order to obviate damag...

Kim, Y.; Lee, K.; Kim, J.; Kim, J.; Heo, Y.; Wang, H.; Lee, K.; Jung, K., 2015: Application of damage control resuscitation strategies to patients with severe traumatic hemorrhage: review of plasma to packed red blood cell ratios at a single institution. When treating trauma patients with severe hemorrhage, massive transfusions are often needed. Damage control resuscitation strategies can be used for such patients, but an adequate fresh frozen plasma: packed red blood cell (FFP:PRBC) administratio...

Petäjä, J.; Pesonen, E.; Fernández, J.A.; Vento, A.E.; Rämö, O.J.; Griffin, J.H., 1999: Cardiopulmonary bypass and activation of antithrombotic plasma protein C. We hypothesized that antithrombotic plasma-activated protein C plays a defensive antithrombotic role during coronary ischemia and postischemic reperfusion. We evaluated protein C activation during cardiopulmonary bypass and coronary reperfusion in...

Battilocchio, C.; Hawkins, J.M.; Ley, S.V., 2013: A mild and efficient flow procedure for the transfer hydrogenation of ketones and aldehydes using hydrous zirconia. A flow chemistry Meerwein-Ponndorf-Verley (MPV) reduction procedure using partially hydrated zirconium oxide via a machine-assisted approach is reported. The heterogeneous reductive system could be applied to a wide range of functionalized substra...

Pollock, J.L.; Coates, J.D., 2001: Potential for in-situ bioremediation in chlorate contaminated environments. Recent studies demonstrated extensive perchlorate (PCL) contamination of the environment. Microbial attenuation is the most feasible means of remediation, however, little is known about perchlorate-reducing bacteria (ClRB) in contaminated environm...

Hu, F.; Liu, H.; Xu, L.; Li, Y.; Liu, X.; Shi, L.; Su, Y.; Qiu, X.; Zhang, X.; Yang, Y.; Zhang, J.; Li, Z., 2016: Hypoxia-inducible factor-1α perpetuates synovial fibroblast interactions with T cells and B cells in rheumatoid arthritis. Synovial fibroblast hyperplasia, T-cell hyperactivity, B-cell overactivation, and the self-perpetuating interactions among these cell types are major characteristics of rheumatoid arthritis (RA). The inflamed joints of RA patients are hypoxic, wit...

Meyer, A. W., 1930: Malpigi as anatomist. Science New York, 72: 235-238