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PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin


, : PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiology Letters 150(2): 209-217

Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptospordium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections.

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Accession: 002914446

DOI: 10.1111/j.1574-6968.1997.tb10372.x

Download PDF Full Text: PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin


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Motokizawa, Fumiaki, 1996: Odor representation and discrimination in mitral/tufted cells of the rat olfactory bulb. Extracellular single-unit responses to odorants with various properties were recorded from mitral/tufted cells over large areas of the olfactory bulb of anesthetized rats. Each cell was exposed to one stimulus set consisting of five different odor...

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Ingram H.A.P.; Rycroft D.W.; Williams D.J.A., 1974: Anomalous transmission of water through certain peats. Field experiments are described in which seepage tubes were used under variable-head and constant-head conditions. The estimates of "hydraulic conductivity" obtained from humified peat showed time dependence, but they also increased with...

Gray R.W.; Lemann J.Jr; Adams N.D., 1979: The regulation of plasma 1 25 di hydroxy vitamin d concentrations in healthy adults. Norman, A W Et Al (Ed ) Vitamin D: Basic Research And Its Clinical Application: Proceedings Of The 4th Workshop on Vitamin D, Berlin, West Germany, Feb Xxvi+1318p Walter De Gruyter: New York, N Y , Usa; Berlin, West Germany Illus P545-552

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