EurekaMag.com logo
+ Translate

PCR-based molecular discrimination of Verticillium chlamydosporium isolates


, : PCR-based molecular discrimination of Verticillium chlamydosporium isolates. Mycological Research 100(7): 801-809

PCR-based assays were performed to resolve the genetic variation between 28 different isolates of Verticillium chlamydosporium using primers designed to amplify ribosomal internal transcribed spacers (ITS) and intergenic spacers (IGS). Different isolates of V. chlamydosporium were also differentiated using primers matching enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic (REP) elements. Restriction fingerprinting of PCR-amplified ITS products failed to yield intraspecific polymorphism, and different levels of discrimination between V. chlamydosporium isolates were not achieved. However, restriction patterns of ITS products digested with Hae III and HinfI were useful in differentiating between some of the closely relate isolates of V. chlamydosporium, plant pathogenic Verticillium species and some common soil fungi. PCR amplification of IGS was found to be the most sensitive method which enabled the detection of 22 variants within the sample of 28 isolates of V. chlamydosporium and six different plant pathogenic Verticillium species. By using ERIC and REP-PCR fingerprinting, isolates were categorized in 20 and 13 genotypes, respectively. In general, PCR-based procedures can differentiate between closely related isolate of V. chlamydosporium within IGS genotypes. This also could be achieved by ERIC and REP-PCR, and may be considered a rapid tool for the genetic characterization and detection of different isolates of V. chlamydosporium.

Accession: 002914470

DOI: 10.1016/s0953-7562(96)80025-6

Download PDF Full Text: PCR-based molecular discrimination of Verticillium chlamydosporium isolates


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Carder, J.H.; Segers, R.; Butt, T.M.; Barbara, D.J.; Von Mende, N.; Coosemans, J., 1993: Taxonomy of the nematophagous fungi Verticillium chlamydosporium and Verticillium suchlasporium based on secreted enzyme activities and RFLP analysis. Eight isolates of Verticillium chlamydosporium Goddard, which have been recently reclassified into two species and two varieties of each species, were studied for biochemical and genetic evidence to support subdivision of the species aggregate. A...

Van Damme, V.; Ceyssens, I.; Vanhove, W.; Viaene, N., 2002: Evaluation of isolates of Verticillium chlamydosporium as biocontrol agents of Meloidogyne spp. Mededelingen 66(2b): 619-621

Damme, V. van; Ceyssens, I.; Vanhove, W.; Viaene, N., 2001: Evaluation of isolates of Verticillium chlamydosporium as biocontrol agents of Meloidogyne spp. Eleven V. chlamydosporium isolates (Vc10, Vc2M, Vcc600.88, 0015, 0040, 0068.1, 0070, 0072, 0077, 0083, and 0086), of which 8 were obtained from Belgian soils, were screened in the laboratory for the suppression of Meloidogyne spp. In vitro, egg pa...

D.D.yn, G.; Derycke, T.; Moens, M.; Viaene, N., 2000: Selection of Belgian Verticillium chlamydosporium isolates for the control of Meloidogyne javanica in greenhouse crops. Nematology 2(7): 749

Kerry, B.R.; Mauchline, T.H.; Atkins, S.D.; Davies, K.G.; Hirsch, P.R., 2000: Molecular methods to study the rhizosphere interactions of Verticillium chlamydosporium. Journal of Nematology 32(4): 438, December

Crump, D.H.; Irving, F., 1992: Selection of isolates and methods of culturing Verticillium chlamydosporium and its efficacy as a biological control agent of beet and potato cyst nematodes. Chlamydospores of Verticillium chlamydosporium produced on four different culture media showed no significant differences in pathogenicity to Heterodera schachtii. The most effective isolates of the fungus tested gave 75% and 76% control of first...

Sosnowska, D.; Mauchline, T.H.; Bourne, J.M.; Kerry, B.R., 2001: Screening of Polish, other European and tropical isolates of Verticillium chlamydosporium to assess their potential as biological control agents of root-knot nematodes. Three Polish (Vc1, Vc2 and Vc3), four other European (C, E, F and D) and three tropical (Vc10, B and A) isolates of the nematophagous fungus V. chlamydosporium were screened for their ability to colonize the surface of barley roots, produce chlamy...

Braga, Fábio.R.; Araújo, J.V.; Campos, A.K.; Carvalho, Rério.O.; Silva, Aé.R.; Tavela, A.O.; Maciel, A.S., 2007: In vitro observation of the action of isolates of the fungi Duddingtonia flagrans, Monacrosporium thaumasium and Verticillium chlamydosporium on the eggs of Ascaris lumbricoides (Linnaeus, 1758). In vitro action of the nematophagous fungi D. flagrans, M. thaumasium and V. chlamydosporium on eggs of A. lumbricoides was observed. After 7, 10 and 14 days of interaction, the fungus which showed the most promise as a biological control agent ag...

Dhananjay Naik; Rao, M.S.; Lakshmi, R.; Reddy, M.K.; Rahiman, B.A., 2003: Molecular detection of Verticillium chlamydosporium, a nematophagous fungus against root-knot nematode (Meloidogyne incognita). Verticillium chlamydosporium is one of the important fungal biological control agents, which is very effective against root-knot nematode infecting most of the horticultural crops. In order to exploit the full biological control potential of the n...

Sosnowska, D.; Kerry, B., R., 2002: Evaluation of Polish and tropical isolates of the nematophagous fungus Verticillium chlamydosporium Goddard for the control of the root-knot nematode (Meloidogyne incognita Kofoid and White) on tomato plants. The potential of three Polish and two tropical isolates of the nematophagous fungus Verticillium chlamydosporium as a biological control agent for the root-knot nematode Meloidogyne incognita in glasshouse conditions on tomato plants was investiga...