EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR-based molecular discrimination of Verticillium chlamydosporium isolates


, : PCR-based molecular discrimination of Verticillium chlamydosporium isolates. Mycological Research 100(7): 801-809

PCR-based assays were performed to resolve the genetic variation between 28 different isolates of Verticillium chlamydosporium using primers designed to amplify ribosomal internal transcribed spacers (ITS) and intergenic spacers (IGS). Different isolates of V. chlamydosporium were also differentiated using primers matching enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic (REP) elements. Restriction fingerprinting of PCR-amplified ITS products failed to yield intraspecific polymorphism, and different levels of discrimination between V. chlamydosporium isolates were not achieved. However, restriction patterns of ITS products digested with Hae III and HinfI were useful in differentiating between some of the closely relate isolates of V. chlamydosporium, plant pathogenic Verticillium species and some common soil fungi. PCR amplification of IGS was found to be the most sensitive method which enabled the detection of 22 variants within the sample of 28 isolates of V. chlamydosporium and six different plant pathogenic Verticillium species. By using ERIC and REP-PCR fingerprinting, isolates were categorized in 20 and 13 genotypes, respectively. In general, PCR-based procedures can differentiate between closely related isolate of V. chlamydosporium within IGS genotypes. This also could be achieved by ERIC and REP-PCR, and may be considered a rapid tool for the genetic characterization and detection of different isolates of V. chlamydosporium.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002914470

DOI: 10.1016/s0953-7562(96)80025-6

PDF Full Text: PCR-based molecular discrimination of Verticillium chlamydosporium isolates


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Hart, J., 1990: Aberrant blue tits. Isle of Wight Birds, 1989: 72-73

Sappal K. K., 1975: Sclerotinite in certain Palaeozoic coals of Australia. Compte Rendu - Congres International de Stratigraphie et de Geologie du Carbonifere = International Congress on Carboniferous Stratigraphy and Geology

Yamashita, T.; Nishikawa, T.; Kawamura, M.; Fujinaga, K., 1996: Site-directed mutagenesis by PCR. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 41(5): 534-538

Hills, J. L., 1930: Agricultural seed fifteen years of agricultural seed inspection. The outstanding results in the analysis for purity and germination are described for 357 samples of agricultural seed collected from dealers in Vermont in 1930. Surveying 15 yr. of seed inspection work in Vermont, a comparison is made of condition...

Potapov, I. A., 1976: Design of a tub for the perfusion of isolated lymphatic vessels. A bath for the perfusion of the isolated thoracic duct was described. From this method changes in the vascular lumen resulting from the action of various agents in vitro could be assessed in dogs.

Wilkens, N.D.; Bowen, B.B.itler; Rupp, J.A., 2010: Anatomy of a Cambrian sheet sand; depositional environments in the Mount Simon Sandstone. The Mount Simon Sandstone is a quartz arenite dominated basal Cambrian unit present throughout the mid-continental United States. It has been variously interpreted as having been deposited in a tidally dominated nearshore, tidal flat, tidal channe...

Fukui, R.; Poinar, E.I.; Bauer, P.H.; Schroth, M.N.; Hendson, M.; Wang, X.L.; Hancock, J.G., 1994: Spatial colonization patterns and interaction of bacteria on inoculated sugar beet seed. Development and spatial distribution of microcolonies of Pseudomonas spp. and Bacillus subtilis GB03 inoculated singly and in combination on sugar beet (Beta vulgaris) seed were observed with a scanning electron microscope (SEM). SEM examination o...

M.S.engli; M.J.n, 1995: Rheology of rocks and fault models. In this paper the authors studied the rheology of rocks and fault models. Recent experimental results concerning rheological properties of rocks are briefly reviewed, and several existing fault models are discussed, and then some suggestions for f...

Bishop, M.R.; Marchigiani, D.; Kasten Sportes, C.; Castro, K.; Carter, C.; Read, E.; Leitman, S.; Hou, J.; Gress, R.; Fowler, D., 2001: Pre-transplant quantitation of host T-cells as a method to determine adequate host immune depletion for successful engraftment of T-cell depleted allografts after reduced-intensity conditioning. Reduced-intensity (RI; non-myeloablative) conditioning regimens permit engraftment of T-cell replete allografts with varying degrees of donor chimerism. Based on a murine model, we are investigating whether sequential, immune-depleting chemotherap...

Yang, D.; Moran-Mirabal, J.M.; Parlange, J-Yves.; Walker, L.P., 2014: Investigation of the porous structure of cellulosic substrates through confocal laser scanning microscopy. At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic sub...