+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

Applied and Environmental Microbiology 61(6): 2358-2364

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

We compare the kinetics of appearance of supernatant enzyme activities (lignin peroxidase, manganese peroxidase, and cellulase) and gene expression (LIC, mnp, and cbhI gene families and the unique cbhII gene) in Phanerochaete chrysosporium ME446 when grown on four different carbon sources: ball-milled straw, representing the natural substrate lignocellulose; Avicel as a crystalline cellulose; and high and low concentrations of glucose, in all cases with limiting nitrogen. PCR-based technology utilizing pairs of primers specific for particular genes showed that there is differential expression between and within the families. There were a number of instances of mRNA species being present only on a single day, implying tight regulation of lignocellulose degradation at the mRNA level. The patterns of extracellular enzyme activities and mnp and cbh gene expression are similar whereas LIG gene expression can be detected when no corresponding enzyme activity is observed in the extracellular supernatant. The enzyme produced under these conditions is presumably sequestered by the mycelium and is likely to be functionally significant. Another striking result is that cellulose, in the form of Avicel, elicits the expression of three LIG genes for which there is no expression under the same conditions with the other carbon sources.

(PDF 0-2 workdays service: $29.90)

Accession: 002914477

PMID: 7793956

Related references

Lignocellulose degradation by Phanerochaete chrysosporium: gene families and gene expression for a complex process. Molecular Microbiology 19(5): 923-932, 1996

Cytochrome P450 oxidoreductase gene of the white rot fungus Phanerochaete chrysosporium Gene transcription and heterologous expression in E coli and Saccharomyces cerevisiae. Abstracts of the General Meeting of the American Society for Microbiology 103: Q-032, 2003

The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium. Microbiology 154(Pt 2): 491-499, 2008

P450 redox enzymes in the white rot fungus Phanerochaete chrysosporium: gene transcription, heterologous expression, and activity analysis on the purified proteins. Current Microbiology 61(4): 306-314, 2011

Isozyme specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin peroxidase genes of Phanerochaete chrysosporium. Bio/Technology 11(7): 830-834, 1993

Gene transcription, heterologous expression, and purification of p450 redox proteins of the white rot fungus Phanerochaete chrysosporium. 2006

Isolation, characterization, and analysis of the expression of the cbhII gene of Phanerochaete chrysosporium. Applied and Environmental Microbiology 60(12): 4387-4393, 1994

Quantitative proteomic analysis of lignocellulolytic enzymes by Phanerochaete chrysosporium on different lignocellulosic biomass. Journal of Proteomics 75(5): 1493-1504, 2012

The green fluorescent protein gene functions as a reporter of gene expression in Phanerochaete chrysosporium. Applied and Environmental Microbiology 67(2): 948-955, 2001

Gene expression analysis of Phanerochaete chrysosporium during the transition time from primary growth to secondary metabolism. Journal of Microbiology 47(3): 308-318, 2009