+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families

, : PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families. Applied and Environmental Microbiology 61(6): 2358-2364

We compare the kinetics of appearance of supernatant enzyme activities (lignin peroxidase, manganese peroxidase, and cellulase) and gene expression (LIC, mnp, and cbhI gene families and the unique cbhII gene) in Phanerochaete chrysosporium ME446 when grown on four different carbon sources: ball-milled straw, representing the natural substrate lignocellulose; Avicel as a crystalline cellulose; and high and low concentrations of glucose, in all cases with limiting nitrogen. PCR-based technology utilizing pairs of primers specific for particular genes showed that there is differential expression between and within the families. There were a number of instances of mRNA species being present only on a single day, implying tight regulation of lignocellulose degradation at the mRNA level. The patterns of extracellular enzyme activities and mnp and cbh gene expression are similar whereas LIG gene expression can be detected when no corresponding enzyme activity is observed in the extracellular supernatant. The enzyme produced under these conditions is presumably sequestered by the mycelium and is likely to be functionally significant. Another striking result is that cellulose, in the form of Avicel, elicits the expression of three LIG genes for which there is no expression under the same conditions with the other carbon sources.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002914477

PMID: 7793956

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ali, O.; Rampal, K. G.; Lubis, S. H., 1984: Nutrition and health components of the Applied Nutrition Programme in Kuala Selangor district. A questionnaire-interview method was used to assess nutrition and health of children under 6 years old in 2 villages in Kuala Selangor, Malaysia; weight was also recorded. One village had taken part in an Applied Nutrition Programme (ANP) for the...

Chukhrov F. V., 1974: The formation of ores from vadose solutions. Geologiya Rudnykh Mestorozhdeniy 16(3): 3-14

Haladus Andrzej; Kulma Ryszard, 1997: Modeling of ground water flow conditions in fault zones. Gospodarka Surowcami Mineralnymi: , Pages 273-278. 1997.

Nakamura, K.; Masuda, Y.; Nakatsujl, K.; Hirooka, T.; Fujimoto, K., 1965: Effects of 2-methoxymethyl-17a-methylestradiol-3-methyl ether on experimental hypercholesterolemia and atherosclerosis in animals. Hypocholesterolemic and anti-atherosclerotic effects of 2-methoxymethyl-17a-methylestradiol-3-methyl ether (P-5780) were studied on the Triton-induced hyperlipidemic rats, cholesterol-fed rats arid rabbits. Prevention of hyperlipidemia caused by T...

Tokumoto, T., 2012: Identification of membrane progestin receptors (mPR) in goldfish oocytes as a key mediator of steroid non-genomic action. One of the most extensively investigated and well characterized models of nongenomic steroid actions initiated at the cell surface is the induction of oocyte maturation (OM) in fish and amphibians by progestin. Gonadotropin induces the final phase...

Haddad, N.G.; Croffie, J.M.; Eugster, E.A., 2004: Pancreatic enzyme elevations in children with diabetic ketoacidosis. The incidence of pancreatic enzyme elevations in children with diabetic ketoacidosis (DKA) compared with children with newly diagnosed diabetes without DKA was assessed in a prospective study. Pancreatic enzyme elevations, particularly hyperlipase...

Kosa, Ferenc, 2000: Application and role of anthropological research in the practice of forensic medicine. Whereas in anthropology the application of the new methods is predominantly taken at random and is characteristic of basic research, in forensic medicine the objectives, motives and results of osteological research are of prime interest, due to it...

Curda D.; Poulsen K.P., 1978: Oxidation of lard at low temperatures. In the introductory part the main factors are discussed which could cause the anomaly and in some cases even reverse course of the temperature dependence for oxidation of lipids.The experimental work demonstrates that the velocity of oxidation exp...

Yushin, V. V., 1986: Fine structure of the cuticle of 3 marine nematode species. The 4 layers (epi-, exo-, meso-, and endocuticle) which form the cuticle of Paracanthonchus macrodon, Axonolaimus seticaudatus and Sabatieria sp. are described from transmission electron microscope studies, and are represented schematically.

Hamann, Otto., 1907: Bronns Klasseu und Ordnungen des Tier-Reichs, wissenschaftlich dargestellt in Wort und Bild Vol II, pt 3, Echinodermen Stachelhauter Book 5 Die Seelilien. Unknown