EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

PCR-mediated differential display and cloning of a melanocyte gene decreased in malignant melanoma and up-regulated with sensitization to DNA damage






Dna and Cell Biology 15(5): 423-427

PCR-mediated differential display and cloning of a melanocyte gene decreased in malignant melanoma and up-regulated with sensitization to DNA damage

Characterization of genes expressed in normal cells and decreased in their malignant counterparts is important for detecting candidate tumor suppressor genes. We have now used comparative differential display of mRNAs from B16 melanoma and matched syngeneic normal melanocytes to detect a G0A gene expressed preferentially in resting G-0 melanocytes compared to proliferating cells. Cloning and sequencing revealed no homology of G0A in the GenBank Database, suggesting that this is a new gene. Northern blot analysis with the cloned probe, confirmed about a five-fold higher expression in normal melanocytes compared to melanoma. Up-regulation of this gene was not detected by L-tyrosine induction of B16 melanoma terminal differentiation, but was seen in these cells, when exposed to the radiation sensitizer bromodeoxyuridine and subsequent UV radiation. Our differential expression data suggest that the G0A gene is important for melanocytic growth control and for the response of melanoma cells to radiation sensitizers.


Accession: 002914478

PMID: 8924217

DOI: 10.1089/dna.1996.15.423



Related references

Cloning of DLM-1, a novel gene that is up-regulated in activated macrophages, using RNA differential display. Gene (Amsterdam) 240(1): 157-163, Nov 15, 1999

Identification and cloning of a developmentally regulated Cryptosporidium parvum gene by differential mRNA display PCR. Gene 216(2): 327-334, 1998

Differential display mediated cloning of anthocyanidin reductase gene from tea (Camellia sinensis) and its relationship with the concentration of epicatechins. Tree Physiology 29(6): 837-846, 2009

Differential sensitivity of thoracic malignant tumors to adenovirus-mediated drug sensitization gene therapy. Journal of Thoracic and Cardiovascular Surgery 109(4): 626-30; Discussion 630-1, 1995

Cloning oncogenic ras-regulated genes by differential display. Methods 16(4): 365-372, 1999

Identification and cloning of RA-regulated genes by mRNA-differential display. Methods in Molecular Biology 89: 389-404, 1998

Analysis of differential gene expression in the kidney by differential cDNA screening, subtractive cloning, and mRNA differential display. Seminars in Nephrology 15(1): 29-42, 1995

Molecular cloning of five stress-regulated genes from sunflower by differential display PCR. Plant Biology (Rockville) : 117, 1999

Cloning differentially regulated genes from chondrocytes using agarose gel differential display. Biochimica Et Biophysica Acta. 1396(3): 237-241, Ch 13, 1998

Cloning regulated genes from differentiating chondrocytes by agarose gel differential display. Bone (New York) 20(4 SUPPL ): 80S, 1997