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PCR-specific detection of a plum pox virus (PPV) isolate in Chile


, : PCR-specific detection of a plum pox virus (PPV) isolate in Chile. Agricultura Tecnica Santiago 56(2): 89-98

A detection method for plum pox potyvirus was optimized on the basis of polymerase chain reaction (PCR). Viral RNA present in a crude plant extract was first processed with reverse transcriptase to produce a cDNA that was used as a template for the PCR reaction, using specific primers designed to anneal at the 3' end of the viral genome.

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