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Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae

, : Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae. Proceedings of the National Academy of Sciences of the United States of America1, 93(12): 6181-6185

The lack of efficient mechanisms for stable genetic transformation of medically important insects, such as anopheline mosquitoes, is the single most important impediment to progress in identifying novel control strategies. Currently available techniques for foreign gene expression in insect cells in culture lack the benefit of stable inheritance conferred by integration. To overcome this problem, a new class of pantropic retroviral vectors has been developed in which the amphotropic envelope is completely replaced by the G glycoprotein of vesicular stomatitis virus. The broadened host cell range of these particles allowed successful entry, integration, and expression of heterologous genes in cultured cells of Anopheles gambiae, the principle mosquito vector responsible for the transmission of over 100 million cases of malaria each year. Mosquito cells in culture infected with a pantropic vector expressing hygromycin phosphotransferase from the Drosophila hsp70 promoter were resistant to the antibiotic hygromycin B. Integrated provirus was detected in infected mosquito cell clones grown in selective media. Thus, pantropic retroviral vectors hold promise as a transformation system for mosquitoes in vivo.

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Accession: 002914869

DOI: 10.2307/39696

PDF Full Text: Pantropic retroviral vectors integrate and express in cells of the malaria mosquito, Anopheles gambiae

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Carp, C., 1978: Value of the electrocardiographic stress test by atrial pacing in the healing stages of experimental myocardial infarction. To check up the value of atrial pacing (AP) in estimating the degree of healing in myocardial necrosis, an ECG study was carried out in 20 dogs with experimental myocardial infarction induced by ligation of the left descending coronary artery. The...

Kroll, G.E.k, D.K.tner, M., 1977: Investment as a goal for the best economical use of funds in dairy combines. Kooperation 11 (8) 350-352

Van Deelen, T.R.; Campai, H.I..; Hamady, M.; Haufler, J.B., 1996: Longevity of wild White-tailed Deer, Odocoileus virginianus, does in Michigan. In conjunction with a telemetry study of White-tailed Deer in northern Michigan, we aged 110 wild adult deer (96 does, 14 bucks) by means of cementum annuli analysis of the incisorform canine tooth. Aging and subsequent radio-tracking suggested th...

Normansell, D.E., 1987: Use of kappa and lambda chain quantitation for the detection of immunoglobulin abnormalities in serum. Quantitation of kappa and lambda light chains was investigated as an alternative to immunoelectrophoresis or immunofixation in the analysis of sera for abnormal immunoglobulins. Normal values for the ratios of total immunoglobulins to total light...

Bardzik, J.; Przeździak, J.; Bardzik, I., 1975: Immunoglobulins in persons with long-term exposure to halothane. In 18 persons (anaesthetists and anaesthetic nurses) with a history of long-term exposure to low concentrations of halothane the activity of aminotransferases, GGTP, and alkaline phosphatase, the bilirubin level and thymol turbidity test were dete...

Meyer-Rüsenberg, H-W., 2007: The suitability of hyaluronic acid in the therapy of deep upper lid sulcus in the anophthalmic orbit. For correcting deep persisting upper lid sulcus in cases of enucleation or evisceration, a subperiostal implant can be used to lift the entire contents of the orbit, or dermis fat can be implanted into the lid. However, there is no optimal surgica...

Zemskov V.M.; Ivanov G.G.; Borisova A.M.; Zakharkin V.A., 1982: Delayed type hyper sensitivity to human heart myosin in patients with rheumatic fever. Highly purified myosin obtained from the muscle of normal human heart left ventricle by special treatment was used as test antigen in the leukocyte migration inhibition test (LMIT) in patients with rheumatic fever. The intensity of the LMIT corres...

Kan, D.V.; Segal, A.S.; Mazo, V.E.; Skarunskiĭ, I.A., 1982: Current diagnostic methods in Peyronie's disease. Urologiia i Nefrologiia: 34-38

Wyse, Donald L., 1992: Future impact of crops with modified herbicide resistance. Weed Technology 6(3): 665-668

Capreol, S.V.; Sutherland, L.E., 1968: Comparative morphology of juxtaglomerular cells. 1. Juxtaglomerular cells. Canadian Journal of Zoology, 46: 249-256