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Response of Hyoscyamus callus cultures to sodium chloride and sodium sulphate salinity

Response of Hyoscyamus callus cultures to sodium chloride and sodium sulphate salinity

Annals of Agricultural Science Cairo 40(2): 515-524

Callus cultures were established from seeds of H. muticus by culturing them on MS medium supplemented with 1 mg 2,4-D + 1 mg kinetin/litre. Calluses were subcultured several times to obtain a stock. Pieces of stock callus were then transferred to basal MS medium containing various concentrations of sodium chloride or sodium sulfate (0.0, 0.3, 0.6, 0.9, 1.2 or 1.5%). Data were recorded after 4 weeks of incubation.

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