Section 3
Chapter 2,965

Standardization of enzyme-linked immunosorbent assays (ELISAs) for quantitative estimation of antibodies specific for infectious bovine rhinotracheitis virus, respiratory syncytial virus, parainfluenza-3 virus, and bovine viral diarrhea virus

Graham, D.A.; Mawhinney, K.A.; McShane, J.; Connor, T.J.; Adair, B.M.; Merza, M.

Journal of Veterinary Diagnostic Investigation Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 9(1): 24-31


ISSN/ISBN: 1040-6387
PMID: 9087921
DOI: 10.1177/104063879700900105
Accession: 002964157

For each test, serum samples were titrated and their end point titres calculated by an algebraic method directly from a plot of each titration series and also from a regression line fitted to this plot. The corrected optical density (COD) of each sample at dilutions of 1/25, 1/50, and 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate. This value was the sample/positive (S/P) ratio.

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