EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Survival and regeneration of dormant silver birch buds stored at super-low temperatures


Canadian Journal of Forest Research 26(4): 617-623
Survival and regeneration of dormant silver birch buds stored at super-low temperatures
Cryopreservation was developed for the storage of in vivo buds of silver birch (Betula pendula Roth). The principles of the optimized method were the use of buds already acclimated in nature and slow freezing with a cooling velocity of 10 degree C/h down to a terminal temperature of -38 degree C. After 24 h at this temperature the buds were immersed in liquid nitrogen at - 196 degree C for 8 days, 6 months, or 12 months. After fast thawing, 5 min in a water bath at 37 degree C, the buds were surface sterilized and cultured according to the normal laboratory routine. The buds were examined after 2 and 4 weeks of cultivation. There were no significant differences in survival and growth between the unfrozen controls and buds stored in liquid nitrogen for different times. The growth percentage of buds without a female catkin was double that of buds with a catkin. These results indicate that cryopreservation would be an ideal method for the ex situ gene conservation of birch and retention of the juvenility of adult genotypes.

Accession: 002973044

DOI: 10.1139/x26-071

Download PDF Full Text: Survival and regeneration of dormant silver birch buds stored at super-low temperatures



Related references

In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures. Biologia Plantarum 49(2): 281-284, 2005

The abscisic acid content of dormant birch buds. Planta 123(3): 291-298, 1975

Farmland afforestation by natural regeneration and direct seeding of hairy birch and silver birch. Farmland afforestation by natural regeneration and direct seeding of hairy birch and silver birch: 75 + 29 pp., 1994

In vitro propagation of the endangered Virginia roundleaf birch (Betula uber) using dormant buds. Forest Science 36(3): 842-846, 1990

In vitro propagation of the endangered virginia roundleaf birch betula uber ashe fern. using dormant buds. Forest Science 36(3): 842-846, 1990

In nature dormant buds and in vitro dormant-like buds of Fraxinus excelsior L. Protoplasma 190(1-2): 16-24, 1996

Super cooling of water in dormant peach flower buds the importance of the bud axis. Plant Physiology (Rockville) 69(4 SUPPL): 120, 1982

Vascular development in dormant prunus flower buds and its relationship to super cooling. Hortscience 17(5): 790-791, 1982

Regeneration of blue honeysuckle via dormant axillary buds. 2008

Structural dynamics of silver birch I. The fates of buds. Journal of Applied Ecology 19(1): 203-218, 1982