+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Survival of free and alginate-encapsulated Pseudomonas aeruginosa UG2Lr in soil treated with disinfectants



Survival of free and alginate-encapsulated Pseudomonas aeruginosa UG2Lr in soil treated with disinfectants



Journal of Applied Bacteriology 80(1): 19-25



Pseudomonas aeruginosa UG2Lr, a rifampicin-resistant strain possessing the luxAB on a chromosomal Tn5 insert, was inoculated into soil microcosms as either free cells or encapsulated in dry alginate beads. A 100-fold increase in cell number g-1 dry soil was observed microcosms inoculated with alginate-encapsulated UG2Lr after 3 weeks incubation at 22 degrees C compared to microcosms inoculated with free cells. After 98 d, microcosms inoculated with free UG2Lr cells contained 10(4) cfu g-1 dry soil compared to 10(7) cfu g-1 dry soil in microcosms inoculated with alginate-encapsulated UG2Lr cells. The effects of disinfectants on both the free and alginate-encapsulated UG2Lr cells were also examined. 1.0% (w/g dry soil) calcium hypochlorite, formaldehyde and Spectrum Clear Bath, were added to microcosms each week for 4 weeks. Formaldehyde killed both free and alginate-encapsulated UG2Lr cells within 14 d after only two amendments. Calcium hypochlorite reduced free UG2Lr cell numbers 10-fold 2 d after initial application; however, the introduced population recovered and was unaffected by subsequent treatments at 7, 14 and 21 d. Alginate-encapsulated UG2Lr cells were not affected by calcium hypochlorite treatment. Spectrum Clear Bath did not kill either free or alginate-encapsulated UG2Lr cells in soil. Alginate encapsulation improved survival of introduced bacteria in soil except in the presence of formaldehyde. Killing genetically-engineered bacteria in soil may be difficult unless a powerful disinfectant such as formaldehyde is used or the genetically-engineered micro-organism is allowed to become non-viable over time.

(PDF emailed within 0-6 h: $19.90)

Accession: 002973137

Download citation: RISBibTeXText

DOI: 10.1111/j.1365-2672.1996.tb03184.x


Related references

Transport and survival of alginate-encapsulated and free lux-lac marked Pseudomonas aeruginosa UG2Lr cells in soil. FEMS microbiology ecology 26(1): 51-61, 1998

Survival of lac-lux marked Pseudomonas aeruginosa UG2Lr cells encapsulated in K-carrageenan and alginate. Journal of Microbiological Methods 23(3): 281-290, 1995

Survival of kappa-carrageenan-encapsulated and unencapsulated Pseudomonas aeruginosa UG2Lr cells in forest soil monitored by polymerase chain reaction and spread plating. FEMS Microbiology Ecology 16(1): 71-82, 1995

Survival and activity of lac-lux marked Pseudomonas aeruginosa UG2Lr cells encapsulated in kappa-carrageenan over four year at 4 degrees C. Journal of Microbiological Methods 30(2): 167-170, 1997

Nutrient-enhanced survival of and phenanthrene mineralization by alginate-encapsulated and free Pseudomonas sp. UG14Lr cell in creosote-contaminated soil slurries. Applied Microbiology & Biotechnology 43(5): 946-951, 1995

Importance of DNase and alginate lyase for enhancing free and liposome encapsulated aminoglycoside activity against Pseudomonas aeruginosa. Journal of Antimicrobial ChemoTherapy 64(2): 317-325, 2009

Survival of alginate-encapsulated Pseudomonas fluorescens cells in soil. Applied Microbiology & Biotechnology 39(4-5): 637-643, 1993

Bioluminescent Most-Probable-Number Method To Enumerate lux-Marked Pseudomonas aeruginosa UG2Lr in Soil. Applied and Environmental Microbiology 60(9): 3458-3461, 1994

Survival of, and root colonization by, alginate-encapsulated Pseudomonas fluorescens cells following introduction into soil. Biology & Fertility of Soils 14(1): 14-22, 1992

Effect of alginate encapsulation and selected disinfectants on survival of and phenanthrene mineralization by Pseudomonas sp UG14Lr in creosote-contaminated soil. Journal of Industrial Microbiology 16(1): 62-67, 1996