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Suspension and protoplast culture of U.S. rice cultivars

Suspension and protoplast culture of U.S. rice cultivars

Plant Cell Reports 15(1-2): 34-37

Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus induction medium for 10-20 weeks without subculture. Weekly subculture of the suspensions for five to eight weeks yielded cells suitable for protoplast isolation. After 2 weeks, rate of colony formation from protoplasts varied among the cultivars and ranged from 2.5 to 6.8%. Improvement of plating efficiencies to as high as 13.7% was obtained by conducting a second cycle of protoplast culture. A total of 525 plants were regenerated from the cultivars studied.

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Accession: 002973489

Download citation: RISBibTeXText

PMID: 24185650

DOI: 10.1007/BF01690249

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