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Suspension culture and single cell regeneration of Pinellia pedatisecta Schott

Suspension culture and single cell regeneration of Pinellia pedatisecta Schott

Acta Agronomica Sinica 22(2): 197-201

Granular embryogenic calluses were produced from mature embryos of this medicinal plant on MS medium supplemented with 2.0 mg 2,4-D and 0.5 mg benzyladenine (BA)/litre. These calluses were suspended in MS medium containing 2.0 mg 2,4-D, 0.1 mg BA and 300 mg casein hydrolysate/litre to give single cell suspensions after 3-4 subcultures. With growth of cells in culture, there was an increase in the content of soluble proteins and a decrease in pH.

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