+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Sustained growth of Ichthyophthirius multifiliis at low temperature in the laboratory

Sustained growth of Ichthyophthirius multifiliis at low temperature in the laboratory

Journal of Parasitology 81(6): 1022-1024

Applied and basic research on the ciliate Ichthyophthirius multifiliis, an obligate parasite of freshwater fishes, requires passage on fish hosts to maintain laboratory stocks. However, continual repeated passage results in senescence of parasite clones over time. Because growth and development are directly correlated to water temperature, our objective was to grow the parasite at low temperature in order to extend the period that the organism remains on the fish, thus reducing: (1) the number of passages and (2) the number of fish required to maintain the parasite over time. Lowering of water temperature from 25 C to 9 C resulted in significant slowing of growth on channel catfish (parasites remained on fish for 20.4 days at 9 C, as compared to 5-6 days at 25 C), with no discernible changes in viability, antigenicity, or infectivity. Low-temperature growth is proposed as an improved method for stable maintenance of I. multifiliis cultures in the laboratory.

(PDF emailed within 0-6 h: $19.90)

Accession: 002973857

Download citation: RISBibTeXText

PMID: 8544046

DOI: 10.2307/3284065

Related references

Observation of laboratory infection by carp by ichthyophthirius multifiliis. Bamidgeh 25(1): 3-9, 1973

Observation of laboratory infection of carp by Ichthyophthirius multifiliis Foquet. Bamidgeh, 251: 3-9, 1973

On the Reproduction of Ichthyophthirius multifiliis Fouquet in Relation to Water Temperature. Nippon Suisan Gakkaishi 3(5): 265-272, 1935

Effects of oral administration of whole plants of Artemisia annua on Ichthyophthirius multifiliis and Aeromonas hydrophila after parasitism by I. multifiliis. Parasitology Research 116(1): 91-97, 2016

New control methods for ichthyophthirius in ponds ichthyophthirius multifiliis ictalurus punctatus malachite green anti parasit. FAO (Food & Agriculture Organization of the United Nations) Fisheries Reports 44(5): 389-392, 1968

Treatment of rainbow trout (Oncorhynchus mykiss Walb.) fry infected with ichthyophthirius (Ichthyophthirius multifiliis) by oral administration of dimetridazole. Bulletin of the European Association of Fish Pathologists 15(2): 67-69, 1995

Temperature- and salinity-dependent development of a Nordic strain of Ichthyophthirius multifiliis from rainbow trout. Journal of Applied Ichthyology 17(6): 273-276, 2001

Temperature-dependent protection against Ichthyophthirius multifiliis following immunisation of rainbow trout using live theronts. Diseases of Aquatic Organisms 72(3): 269-273, 2006

Infection with trophozoites of Ichthyophthirius multifiliis (Ciliophora) in Poecilia vivipara (Poeciliidae) as an experimental host FT Infeccao com trofozoitos de Ichthyophthirius multifiliis (Ciliophora) em Poecilia vivipara (Poeciliidae) como hospedeiro experimental. 2007

Antibody mediated immune response against Ichthyophthirius multifiliis using excised skin from channel catfish, Ictalurus punctatus (Rafinesque), immune to Ichthyophthirius. Journal of Fish Diseases 25(5): 299-306, 2002

Observations on Ichthyophthirius multifiliis in relation to growth performance of rainbow trout fry Salmo gairdneri (Richardson). Pakistan Journal of Zoology 24(3): 187-190, 1992

Influence of Ichthyophthirius multifiliis mucus infection on growth of rainbow trout, Salmo gairdneri (Richardson). Pakistan Journal of Zoology 29(4): 411-413, 1997

Temperature effects on immune response and hematological parameters of channel catfish Ictalurus punctatus vaccinated with live theronts of Ichthyophthirius multifiliis. Fish and Shellfish Immunology 31(6): 774-780, 2012

Ichthyophthirius multifiliis Protozoa in acari, Ancistrus spp Pisces Teleostei, captured in Belem, State of Para Infeccao por Ichthyophthirius multifiliis Protozoa em acari, Ancistrus spp Pisces Teleostei, capturado em Belem, Estado do Para. Revista Brasileira de Ciencia Veterinaria. Janeiro-Abril; 51: 9-10, 1998

The ultrastructure of ichthyophthirius multifiliis. Proceedings of the Pennsylvania Academy of Science 51(1): 98, 1977