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Sweet potato feathery mottle potyvirus (C1 isolate) virion and RNA purification



Sweet potato feathery mottle potyvirus (C1 isolate) virion and RNA purification



Journal of Virological Methods 44(1): 109-116



A procedure for the purification of a Peruvian isolate (C1) of sweet potato feathery mottle potyvirus (SPFMV) and infective RNA has been developed. The use of Hepes [N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid] buffer containing urea and sodium EDTA as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from Nicotiana benthamiana L. Domin. A short RNA isolation procedure yielded infectious RNA, from which ds cDNA of nearly genome size could be obtained. Sweet potato feathery mottle potyvirus, Purification, RNA isolation, cDNA synthesis.

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Accession: 002973949

Download citation: RISBibTeXText

PMID: 8227274

DOI: 10.1016/0166-0934(93)90013-h


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