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The case of chicken attachment region binding protein reinforces the importance of pre-screening of several libraries for cDNA cloning


, : The case of chicken attachment region binding protein reinforces the importance of pre-screening of several libraries for cDNA cloning. Biochemical Journal 321: 261-262



Accession: 002980020

PMID: 9003428

DOI: 10.1042/bj3210261u

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Related references

Qian, Z.; Wilusz, J., 1993: Cloning of a cDNA encoding an RNA binding protein by screening expression libraries using a northwestern strategy. Successful cloning with cDNA expression libraries involves interaction of ligand molecules (probes) with expressed fusion proteins of interest. So far those ligands leading to successful results fall into three classes: (i) antibodies, (ii) protei...

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Matthias P.; Mueller M.M.; Schaffner W., 1991: Cloning of sequence specific dna binding proteins by screening lambda cdna expression libraries with radiolabelled binding site probes. Jost, J P And H P Saluz (Ed ) Biomethods, Vol 5 A Laboratory Guide to in Vitro Studies Of Protein-Dna Interactions 326p Birkhaeuser Verlag: Basel, Switzerland; Boston, Massachusetts, Usa Illus 233-244

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Matuoka, K.; Shibata, M.; Yamakawa, A.; Takenawa, T., 1992: Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries. The protein ASH (for abundant Src homology), composed of 1 Src homology region (SH) 2 and 2 SH3 domains, was cloned by screening human and rat cDNA libraries with an oligonucleotide probe directed to a consensus sequence of the SH2 domains. The ra...

Yonemura, I.; Watanabe, T.; Miyazaki, J.I.; Hirabayashi, T., 1996: Cloning of chicken slow-muscle-type troponin T cDNA Cloning of 5 region cDNA using the 5-RACE method. Zoological Science (Tokyo) 13(SUPPL ): 48

Okayama, H.; Kawaichi, M.; Brownstein, M.; Lee, F.; Yokota, T.; Arai, K., 1987: High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells. Methods in Enzymology 154: 3-28

Srikantha, T.; Landsman, D.; Bustin, M., 1988: Cloning of the chicken chromosomal protein HMG-14 cDNA reveals a unique protein with a conserved DNA binding domain. The isolation and sequencing of a cDNA clone coding for the entire sequence of the protein is described. The open reading frame constitutes only 25% of the transcript, the 5'-untranslated region is extremely rich in GC residues, and the 3&apo...

Weitzel, J.M.; Straetling, W.H., 1997: The MAR binding domain of chicken attachment region binding protein. European Journal of Cell Biology 72(SUPPL 43): 81