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The cdc2Ms Kinase Is Differently Regulated in the Cytoplasm and in the Nucleus


, : The cdc2Ms Kinase Is Differently Regulated in the Cytoplasm and in the Nucleus. Plant Physiology 113(3): 841-852

To study a cyclin-dependent kinase (CDK) from alfalfa (Medicago sativa L.), an antibody was raised against the C-terminal 16 amino acids of the protein cdc2aMs. The cdc2Ms protein was immunopurified with this antibody and its histone kinase activity was measured. The cdc2Ms kinase is activated at the G1/S transition when phosphate-starved cells from the G0 phase re-enter the cell cycle and remain active as cells transit the S, G2, and M phases, indicating that the same CDK regulates all of these phases in alfalfa. In contrast, when cdc2Ms kinase was purified by binding to p13suc1, it was active only in the G2 and M phases. In immunoblots the C-terminal antibody detected an equal amount of the cdc2Ms protein in the cytoplasm and in the nucleus. By indirect immunofluorescence, however, the cytoplasmic form of cdc2Ms could not be found in the S phase of the cells, indicating that the epitope for the cdc2 antibody is not accessible. Binding of putative inhibitor proteins to cdc2 was shown by inactivation of purified plant CDK when cell extracts were added. Furthermore, purified CDK inhibitors, such as the mouse p27kip1 and the yeast p40sic1, blocked the purified plant CDK activity.

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Accession: 002980054

PMID: 12223648

DOI: 10.2307/4277599

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Misaki T.; Allwork S.P.; Bentall H.H., 1983: Long term effects of cryo surgery in the sheep heart. The long-term effects of both epicardial and endocardial cryosurgery were studied with particular reference to the coronary arteries. Sheep were subjected to epicardial cryosurgery without cardiopulmonary bypass, and to endocardial application wit...

Aksenovich, T. I.; Ginzburg, E. Kh, 1993: Planned sampling for analysis of linkage. Incomplete penetrance. For linkage between 2 loci, the effect of degree of penetrance on pedigree sample sizes required to establish linkage with a given degree of accuracy was determined. Two methods of increasing the information content of a sample are discussed, and...

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Mccormick P.A.; Chin J.; Dick R.; Greenslade L.; Biagini M.R.; Cardin F.; Mcintyre N.; Burroughs A.K., 1992: Octreotide and splanchnic haemodynamics in portal hypertension. Journal of Hepatology 16(SUPPL 1): S102

Erikson, D.; Montgomery, H. B. S., 1945: Certain aspects of resistance of Plum trees to bacterial canker. Part III. The action of cell-free filtrates of Pseudomonas morspronorum (Wormald) and related phytopathogenic bacteria on Plum trees. The authors describe experiments designed to show whether the destruction of host cells before their penetration by Pseudomonas morsprunorum is due to the production of a toxin by the organism. Four injections of cell-free filtrates, each containi...

Chang, Hyun Ah; Choi, Keum Hwa; Oh, Tae Kwon; Kwon, Ae Ran; Kim, Dong Hyun; Choi, Eung Chil, 1998: Antibiotic susceptibility of Bifidobacterium spp. strains isolated from healthy Korean. Minimal inhibitory concentrations (MICs) of Bifidobacterium spp. strains (Bifidobacterium breve K-110. B. breve K-111 and B. infantis K-525) isolated from healthy Korean against antituberculosis agents and fluoroquinolones were determined. From th...

Peck, A. E., 1979: Reflections of hedging on futures market activity. The commonly accepted proposition that levels of activity on futures markets reflect commercial as distinct from speculative needs is examined. Direct testing of the relationship between commercial use and total market activity provides a useful s...

Szollosi, D., 1971: Morphological changes in mouse eggs due to aging in the fallopian tube. Changes become evident after unfertilized eggs have spent approx. 12 hr in the oviduct. The spindle rotates, and later migrates to the centre of the egg. In most eggs, the spindle is maintained throughout the first 24 hr after ovulation. In some e...

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