EurekaMag.com logo
+ Translate

The cdc2Ms Kinase Is Differently Regulated in the Cytoplasm and in the Nucleus


, : The cdc2Ms Kinase Is Differently Regulated in the Cytoplasm and in the Nucleus. Plant Physiology 113(3): 841-852

To study a cyclin-dependent kinase (CDK) from alfalfa (Medicago sativa L.), an antibody was raised against the C-terminal 16 amino acids of the protein cdc2aMs. The cdc2Ms protein was immunopurified with this antibody and its histone kinase activity was measured. The cdc2Ms kinase is activated at the G1/S transition when phosphate-starved cells from the G0 phase re-enter the cell cycle and remain active as cells transit the S, G2, and M phases, indicating that the same CDK regulates all of these phases in alfalfa. In contrast, when cdc2Ms kinase was purified by binding to p13suc1, it was active only in the G2 and M phases. In immunoblots the C-terminal antibody detected an equal amount of the cdc2Ms protein in the cytoplasm and in the nucleus. By indirect immunofluorescence, however, the cytoplasmic form of cdc2Ms could not be found in the S phase of the cells, indicating that the epitope for the cdc2 antibody is not accessible. Binding of putative inhibitor proteins to cdc2 was shown by inactivation of purified plant CDK when cell extracts were added. Furthermore, purified CDK inhibitors, such as the mouse p27kip1 and the yeast p40sic1, blocked the purified plant CDK activity.

(PDF same-day service: $19.90)

Accession: 002980054

PMID: 12223648

DOI: 10.2307/4277599

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Becraft P.W.; Taylor G.A., 1989: Effects of nucleus cytoplasm and male sterile nucleus cytoplasm combinations on callus initiation in anther culture of wheat. Nuclear and cytoplasmic factors affect tissue culture response in wheat (Triticum aestivum), and cytoplasmic male sterility may enhance callus initiation in anther culture. Three wheat nuclear genotypes, each in normal and two alien cytoplasms con...

Ear, T.; Fortin, C.F.; Simard, Fçois.A.; McDonald, P.P., 2010: Constitutive association of TGF-beta-activated kinase 1 with the IkappaB kinase complex in the nucleus and cytoplasm of human neutrophils and its impact on downstream processes. Neutrophils influence innate and adaptative immunity by generating numerous mediators whose regulation largely depends on the IkappaB kinase (IKK)/IkappaB/NF-kappaB signaling cascade. A singular feature of neutrophils is that they express several...

Lee, S.Hee.; Choi, J.; Kim, H.; Lee, D.Hoon.; Roh, G.Seob.; Kim, H.Joon.; Kang, S.Soo.; Choi, W.Sung.; Cho, G.Jae., 2014: FK506 reduces calpain-regulated calcineurin activity in both the cytoplasm and the nucleus. Excessive immune responses induced by ischemia-reperfusion injury (IRI) are known to lead to necrotic and apoptotic cell death, and calcineurin plays a major role in this process. Calcineurin dephosphorylates the nuclear factor of activated T-cell...

Woo, E.S.; Wang, A.; Lazo, J.S., 1996: Functional consequences of regulated metallothionein expression in the nucleus or cytoplasm of knockout fibroblasts. Proceedings of the American Association for Cancer Research Annual Meeting 37(0): 31

Schröder, E.; Gebel, L.; Eremeev, A.A.; Morgner, J.; Grum, D.; Knauer, S.K.; Bayer, P.; Mueller, J.W., 2012: Human PAPS synthase isoforms are dynamically regulated enzymes with access to nucleus and cytoplasm. In higher eukaryotes, PAPS synthases are the only enzymes producing the essential sulphate-donor 3'-phospho-adenosine-5'-phosphosulphate (PAPS). Recently, PAPS synthases have been associated with several genetic diseases and retroviral i...

Panaretakis, T.; Hjortsberg, L.; Tamm, K.Pokrovskaja.; Björklund, A-Charlotte.; Joseph, B.; Grandér, D., 2007: Interferon alpha induces nucleus-independent apoptosis by activating extracellular signal-regulated kinase 1/2 and c-Jun NH2-terminal kinase downstream of phosphatidylinositol 3-kinase and mammalian target of rapamycin. Interferon (IFN)alpha induces apoptosis via Bak and Bax and the mitochondrial pathway. Here, we investigated the role of known IFNalpha-induced signaling cascades upstream of Bak activation. By pharmacological and genetic inhibition of the kinases...

Campo, L.; Turley, H.; Han, C.; Pezzella, F.; Gatter, K.C.; Harris, A.L.; Fox, S.B., 2005: Angiogenin is up-regulated in the nucleus and cytoplasm in human primary breast carcinoma and is associated with markers of hypoxia but not survival. Angiogenin, a 14.2 kD polypeptide that was originally noted for its angiogenic activity, is now increasingly recognized to have a multiplicity of biological roles in both physiological and pathological conditions. In breast cancer, there are confl...

Yao, Q.; Jin, W-Lin.; Wang, Y.; Ju, G., 2007: Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development. (1) A Little information exists on the distribution of Slit-Robo-GTPase activating proteins (srGAPs), particularly about their intracellular locations, which may provide further clues to their functions. The purpose of this study is to elucidate t...

Mishima, K.; Matsuoka, H.; Nakajima, H.; Yoshikawa, T.; Ohgushi, H.; Tabata, S.; Yamada, E.; Ichijima, K., 1997: Increased expression of extracellular signal regulated kinase 1 after axotomy in the dorsal motor nucleus of the vagus nerve and the hypoglossal nucleus. The extracellular signal regulated kinases (Erks) cascade is a major signalling system by which cells transduce extracellular signals into intracellular responses. To obtain information about the role of Erks in retrograde neuronal reaction, we in...

Ngo, J.L.; Orlando, R.A.; Ibsen, K.H., 1986: Pyruvate kinase and total protein are regulated differently during growth of P-815 mastocytoma cells. Total protein content of P-815 mastocytoma cells decreases and then increases in response to initiation of a new growth cycle. As the level increases, the rate of synthesis declines. Both events occur prior to any decrease in the rate of cellular...