EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The chicken vitellogenin II gene is flanked by a GATA factor-dependent estrogen response unit


Molecular Endocrinology 10(8): 937-944
The chicken vitellogenin II gene is flanked by a GATA factor-dependent estrogen response unit
The chicken vitellogenin II (VTGII) gene is flanked by an imperfect estrogen response element (ERE) at -350 and a perfect ERE at -620. In the present study we show that this imperfect ERE lies within an estrogen response unit (ERU) that requires a GATA factor and the estrogen receptor to function as an estrogen-dependent enhancer. We infer that GATA-6 contributes to the estrogen-dependent and liver-specific regulation of the endogenous VTGII gene since this is the predominant GATA factor expressed in adult liver. Our analysis of the VTGII ERU revealed four salient points. First, this ERU is comprised of an ERE and a bank of functionally redundant GATA-binding sites. Second, the GATA-6 transactivation domain is necessary (and sufficient, when tethered near the ERE) to render this ERU functional. Third, ERU enhancer activity is dependent on GATA 6, regardless of whether the resident ERE is imperfect or perfect. Fourth, in contrast to a report that the estrogen receptor antagonizes the activity of another GATA factor (GATA-1), we show that these two factors can function in a synergistic manner within the context of the VTGII ERU.


Accession: 002980290

PMID: 8843410

DOI: 10.1210/mend.10.8.8843410



Related references

Preferential binding of estrogen-receptor complex to a region containing the estrogen-dependent hypomethylation site preceding the chicken vitellogenin II gene. Proceedings of the National Academy of Sciences of the United States of America 81(2): 429-433, 1984

Estrogen dependent methylation changes at the 5 end of the chicken vitellogenin gene. Journal Of Cellular Biochemistry Supplementt A: 152, 1983

An estrogen-dependent demethylation at the 5' end of the chicken vitellogenin gene is independent of DNA synthesis. Nucleic Acids Research 12(2): 1163-1177, 1984

Chicken vitellogenin gene-binding protein, a leucine zipper transcription factor that binds to an important control element in the chicken vitellogenin II promoter, is related to rat DBP. Molecular and Cellular Biology 11(10): 4863-4875, 1991

Two functional estrogen response elements are located upstream of the major chicken vitellogenin gene. Molecular and Cellular Biology 8(3): 1123-1131, 1988

Chicken ovalbumin upstream promoter-transcription factor interacts with estrogen receptor, binds to estrogen response elements and half-sites, and inhibits estrogen-induced gene expression. Journal of Biological Chemistry 272(50): 31465-31474, 1998

In vivo and in vitro protein dna interactions at the distal estrogen response element of the chicken vitellogenin gene evidence for the same protein binding to this sequence in hen and rooster liver. Journal of Steroid Biochemistry & Molecular Biology 38(2): 275-284, 1991

Estrogen response module of the mouse lactoferrin gene contains overlapping chicken ovalbumin upstream promoter transcription factor and estrogen receptor-binding elements. Molecular Endocrinology 6(3): 355-364, 1992

Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene. Proceedings of the National Academy of Sciences of the United States of America 79(14): 4252-4255, 1982

In vivo footprinting of the estrogen-inducible vitellogenin II gene from chicken. Nucleic Acids Research 16(20): 9663-9676, 1988