EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The Competence of Maize Shoot Meristems for Integrative Transformation and Inherited Expression of Transgenes


, : The Competence of Maize Shoot Meristems for Integrative Transformation and Inherited Expression of Transgenes. Plant Physiology 110(4): 1097-1107

We have developed a novel and reproducible system for recovery of fertile transgenic maize (Zea mays L.) plants. The transformation was performed using microprojectile bombardment of cultured shoot apices of maize with a plasmid carrying two linked genes, the Streptomyces hygroscopicus phosphinothricin acetyltransferase gene (bar) and the potato proteinase inhibitor II gene, either alone or in combination with another plasmid containing the 5[prime] region of the rice actin 1 gene fused to the Escherichia coli [beta]-glucuronidase gene (gus). Bombarded shoot apices were subsequently multiplied and selected under 3 to 5 mg/L glufosinate ammonium. Co-transformation frequency was 100% (146/146) for linked genes and 80% (41/51) for unlinked genes. Co-expression frequency of the bar and gus genes was 57% (29/51). The co-integration, co-inheritance, and co-expression of bar, the potato proteinase inhibitor II gene, and gus in transgenic R0, R1, and R2 plants were confirmed. Localized expression of the actin 1-GUS protein in the R0 and R1 plants was extensively analyzed by histochemical and fluorometric assays.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002980527

PMID: 12226244

DOI: 10.2307/4277092

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Mizoi K.; Takaku A.; Kayama T.; Kodama N.; Suzuki J., 1981: Subdural effusion following total extirpation of a chiasmal region tumor in children 4 cases. Child's Brain 8(1): 75-76

Koller, D.; Bjorkman, O.; Ritter, S., 1995: Role of pulvinar chloroplasts in light-driven leaf movements of the trifoliate leaf of bean (Phaseolus vulgaris L.). Laminar pulvini of bean (Phaseolus vulgaris L.) contain numerous chloroplasts in cells of their motor tissue. The quantitative relationships of the chloroplast pigments, chlorophyll a and b, beta-carotene, lutein, neoxanthin as well as the xanthop...

Koal, T.; Burhenne, H.; Römling, R.; Svoboda, M.; Resch, K.; Kaever, V., 2005: Quantification of antiretroviral drugs in dried blood spot samples by means of liquid chromatography/tandem mass spectrometry. For the first time approved antiretroviral drugs, i.e. protease inhibitors (PI) and non-nucleoside reverse transcriptase inhibitors (NNRTI), were quantified in dried blood spots (DBS) from HIV/ AIDS patient whole blood samples as the basis for the...

Yang Bin; Liu JiKai; Ye JianRen; Bao Hong; Dong ZeJun, 2001: Studies on the separation, purification, structure of LA-II toxin producing by brown spot needle blight fungus (Lecanosticta acicola). Brown spot needle blight, caused by Lecanosticta acicola [Mycosphaerella dearnessii], is an important disease of pine species in South China. The fungal pathogen could produce toxic substances that make the needle wilt. Rp-18 reversed phase silica...

Crosbie, G. B.; Lambe, W. J., 1993: The application of the flour swelling volume test for potential noodle quality to wheat breeding lines affected by sprouting. This study has shown that the flour swelling volume (FSV) test can be applied successfully to severely sprouted wheat grain. This extends the application of the test for discriminating among early generation breeding lines for their potential suit...

Selinger K., 1985: Pharmacokinetics of mexiletine in healthy subjects taking cimetidine. Clinical Researchpart 1: 284a

Noller, J; Ratchford, Cb, 1981: Managing a pre-order food cooperative. C Missouri University Cooperative Extension Service: 81 (943)

Jensen, Bo Skaaning; Christophersen, Palle; Strobaek, Dorte; Teuber, Lene, 2002: Ion channel modulating agents. The present invention relates to ion channel modulating agents. More particularly, the present invention relates to a particular class of oxime derivatives represented by general formula (I) that has proven useful as modulators of SKCa, IKCa and B...

Coleman, R. J.; Russon, L.; Blanshard, K.; Currie, S., 1993: Useless hand of oppenheim Magnetic resonance imaging findings. Postgraduate Medical Journal 69(808): 149-150

Lisohorov, Ks, 1977: Factors affecting the yield of irrigated maize. Zroshuvane zemlerobstvo: 2 29-32