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The Construction and Complementary Expression of two TMV Defective Particles in Tobacco


Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 28(4): 358-366
The Construction and Complementary Expression of two TMV Defective Particles in Tobacco
Two defective mutants of the tobacco mosaic tobamovirus (TMV), TMVRP and TMVCP, were constructed and assembled in vitro. In TMVRP, the 3'-end and part of the TMV coat protein (CP) gene was deleted; in TMRCP, most of the replicase genes were deleted. These mutant particles were separately or complementary inoculated into tobacco protoplasts by electroporation.


Accession: 002980749

PMID: 12232627



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