EurekaMag.com logo
+ Translate

The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains


, : The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains. Biotechnology letters 18(9): 991-996

An inducible marker system suitable for Agrobacterium tumefaciens was constructed to enable detection and enumeration of specific bacterial cells introduced into soil. A BamHI cassette carrying the catechol 2,3-dioxygenase (C230) gene, tdnC, fused to the nopaline-inducible Agrobacterium promoter Pi 2[noc] was constructed. This cassette was introduced into the broad host range vector pDSK5019 resulting in plasmid pTVNC2. Inducible C230 activity was observed in an A. tumefaciens strain carrying the plasmid pTVNC2 when nopaline was present. Colonies of bacteria tagged with the system could easily be identified by spraying agar plates containing nopaline with catechol, which is converted to the bright yellow compound 2-hydroxymuconic semialdehyde. The inducible tdnC cassette has also been introduced into the BamHI site of the transposon Tn5 carried by the pSUP1011 suicide vector which can be used as a delivery system for the stable introduction of the inducible marker into the chromosome of target cells.

Accession: 002980756

DOI: 10.1007/bf00129719

Download PDF Full Text: The construction of a nopaline-inducible marker system for rapid detection of Agrobacterium strains


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Kawalek M.D.; Loper J.E.; Moore L.W.; Stockwell V.O., 1989: Influence of the agrobacterium radiobacter strain k84 nopaline plasmid pat84b on conjugal transfer of plasmids between agrobacterium strains. Phytopathology 79(8): 909-910

Palanichelvam; Veluthambi, 1996: Octopine- and Nopaline-Inducible Proteins in Agrobacterium tumefaciens Are Also Induced by Arginine. Octopine induced the synthesis of 83, 76, 62, 58, 44, 42, 31, and 22 kDa proteins in Agrobacterium tumefaciens strains harboring the tumor-inducing (Ti) plasmids pTiA6 and pTiAch5. Nopaline induced the synthesis of 83, 76, 62, 58, 56, 44, 42, 31,...

Palanichelvam, K.V.luthambi, K., 1996: Octopine- and nopaline-inducible proteins in Agrobacterium tumefaciens are also induced by arginine. Octopine induced the synthesis of 83, 76, 62, 58, 44, 42, 31, and 22 kDa proteins in Agrobacterium tumefaciens strains harboring the tumor-inducing (Ti) plasmids pTiA6 and pTiAch5. Nopaline induced the synthesis of 83, 76, 62, 58, 56, 44, 42, 31,...

Wabiko H.; Kagaya M.; Sano H., 1990: Various nopaline catabolism genes located outside the ti plasmids in agrobacterium tumefaciens strains. The structure and location of nopaline catabolism (noc) genes were examined in various Agrobacterium tumefaciens strains by plasmid transfer and by Southern hybridization analysis. In a pathogenic strain, PyTS3, the noc genes were identified both...

Melchers, L.; Maroney, M.; Dulk-Ras, A.D.n; Thompson, D.; Vuuren, H.-Van; Schilperoort, R.; Hooykaas, P., 1990: Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from t...

Melchers, L.S.; Maroney, M.J.; den Dulk-Ras, A.; Thompson, D.V.; van Vuuren, H.A.; Schilperoort, R.A.; Hooykaas, P.J., 1990: Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from t...

Wostemeyer, A.; Otten, L.A.B.M.; Schell, J.S., 1984: Sexual transmission of T-DNA in abnormal tobacco regenerants transformed by octopine and nopaline strains of Agrobacterium tumefaciens. Transformed shoots regenerating from crown gall tissues were produced by in vitro infection of regenerating tobacco protoplasts with Agrobacterium tumefaciens. As these shoots were abnormal in their growth and were unable to form roots, they were...

Lin B Y., 1979: Unique metabolites in crown gall tumors induced by agrobacterium tumefaciens strains which utilize neither octopine nor nopaline. Plant Physiology (rockville)suppl): 57

Clare, B.G.; Kerr, A.; Jones, D.A., 1990: Characteristics of the nopaline catabolic plasmid in Agrobacterium strains K84 and K1026 used for biological control of crown gall disease. Wild-type Agrobacterium radiobacter strains 84 and its Tra- derivative K1026, used for biological control of crown gall disease, each contain the plasmid pAtK84b. It confers incompatibility to tumor-inducing (Ti) plasmids of pathogenic A. tumefaci...

Kim, M.S.k; Park, J.K.; Eum, J.S.ong; Sim, W.S.op, 1996: Effective combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of plant vector system. The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transforme...