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Chapter 3,006

Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes

Alfano, J.R.; Ham, J.H.; Collmer, A.

Journal of Bacteriology 177(15): 4553-4556

1995

ISSN/ISBN: 0021-9193
PMID: 7635842
Accession: 003005625
Erwinia chrysanthemi mutant CUCPB5047, delta (pellA pelE) delta (pelB pelC)::28bp delta (pelX) delta 4bp pehX::omega Cmr, was constructed, mutated with Tn5tac1, and screened for isopropyl-beta-D thiogalactopyranoside-dependent pectate lyase (Pel) production. A Kmr SacI fragment from the hyperexpressing Pel+ mutant CUCPB5066 was cloned into Escherichia coli and sequenced. The gene identified, pelL, encodes a novel, asparagine-rich, highly alkaline enzyme that is similar in primary structure to PelX and in enzymological properties to PelE.

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Related References

Ried, J.L.; Collmer, A. 1987: Construction and analysis of an erwinia chrysanthemi mutant containing deletions in the pel genes encoding all of the major pectate lyase isozymes Phytopathology 77(12): 1719
Schoedel, C.; Ried, J.L.; Collmer, A. 1984: Subcloning of genes encoding different erwinia chrysanthemi pectate lyase isozymes Phytopathology 74(7): 876-877
Collmer, A.; Bateman, D.F. 1982: Regulation of extracellular pectate lyase in erwinia chrysanthemi evidence that reaction products of pectate lyase and exo poly alpha d galacturonosidase mediate induction on d galacturonan Physiological Plant Pathology 21(2): 127-140
Collmer, A.B.teman, D. 1982: Regulation of extracellular pectate lyase in Erwinia chrysanthemi: evidence that reaction products of pectate lyase and exo-poly-alpha-D-galacturonosidase mediate induction on D-galacturonan in the soft rot diseases Physiological plant pathology 21(2): 127-139
Ried, J.L.; Collmer, A. 1988: Construction and characterization of an Erwinia chrysanthemi mutant with directed deletions in all of the pectate lyase structural genes Molecular Plant Microbe Interactions 1(1): 32-38
Roeder, D.L.; Collmer, A. 1985: Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi Journal of Bacteriology 164(1): 51-56
Lojkowska, E.; Masclaux, C.; Boccara, M.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N. 1995: Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937 Molecular microbiology 16(6): 1183-1195
Payne, J.H.; Schoedel, C.; Keen, N.T.; Collmer, A. 1987: Multiplication and Virulence in Plant Tissues of Escherichia coli Clones Producing Pectate Lyase Isozymes PLb and Ple at High Levels and of an Erwinia chrysanthemi Mutant Deficient in Ple Applied and Environmental Microbiology 53(10): 2315-2320
Temsah, M.; Bertheau, Y.; Vian, B. 1991: Pectate lyase fixation and pectate disorganization visualized by immunocytochemistry in saintpaulia ionantha infected by erwinia chrysanthemi Cell Biology International Reports 15(7): 611-620
Pissavin, C.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N. 1996: Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937 Journal of Bacteriology 178(24): 7187-7196
Laing, E.; Pretorius, I.S. 1993: Co-expression of an Erwinia chrysanthemi pectate lyase-encoding gene (pelE) and an E. carotovora polygalacturonase-encoding gene (peh1) in Saccharomyces cerevisiae Applied Microbiology and Biotechnology 39(2): 181-188
Kelemu, S.; Collmer, A. 1993: Erwinia chrysanthemi EC16 Produces a Second Set of Plant-Inducible Pectate Lyase Isozymes Applied and Environmental Microbiology 59(6): 1756-1761
Atkinson, M.M.; Baker, C.J.; Collmer, A. 1986: Transient Activation of Plasmalemma K Efflux and H Influx in Tobacco by a Pectate Lyase Isozyme from Erwinia chrysanthemi Plant Physiology 82(1): 142-146
Thurn, K.K.; Barras, F.; Chatterjee, A.K. 1986: Cloning of the erwinia chrysanthemi strain ec 16 pel genes biochemical and immunological characterization of the pectate lyase isozymes Abstracts of the Annual Meeting of the American Society for Microbiology 86: 212
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Collmer, A.; Bateman, D.F. 1981: Regulation of endo pectate lyase synthesis in erwinia chrysanthemi evidence for role of oligo galacturonide lyase Phytopathology 71(2): 209-210
Schoedel, C.; Collmer, A. 1986: Evidence of homology between the pectate lyase-encoding pelB and pelC genes in Erwinia chrysanthemi Journal of Bacteriology 167(1): 117-123
Collmer, A.; Bateman, D.F. 1981: Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligogalacturonide lyase Proceedings of the National Academy of Sciences of the United States of America 78(6): 3920-3924
Reverchon, S.; Van Gijsegem, F.; Rouve, M.; Kotoujansky, A.; Robert-Baudouy, J. 1986: Organization of a pectate lyase gene family in Erwinia chrysanthemi Gene 49(2): 215-224