+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A dose-response relationship between exposure to cockroach allergens and induction of sensitization in an experimental asthma in Hartley guinea pigs

A dose-response relationship between exposure to cockroach allergens and induction of sensitization in an experimental asthma in Hartley guinea pigs

Journal of Allergy and Clinical Immunology 101(5): 653-659

Objective: An indoor allergen, cockroach allergen (CRa) has been partly implicated in the rising prevalence of inner-city asthma. This study investigates a dose-response relationship of exposure to CRa with the degree of sensitization and with airway inflammation through the use of our established guinea pig asthma model. Methods: Four groups of Hartley guinea pigs were exposed to aerosolized saline (control) and three dose levels of CRa: low-dose, 0.5 mg (LD); medium-dose, 5 mg (MD); or high-dose, 25 mg (HD), nebulized twice a day, 5 days per week, for 4 weeks. The development of anaphylactic antibodies was measured by both allergy skin test and passive cutaneous anaphylaxis assay. Animals were challenged with CRa 5 days after the last sensitization, and specific airway resistance was measured continuously by a double-chamber plethysmograph while animals were in the conscious state. The inflammatory cells in bronchoalveolar lavage fluid (BALF) and the contractile responses of tracheal rings were analyzed in vitro at 24 hours after CRa challenge. Results: The anaphylactic antibodies to CRa were detected in the CRa-sensitized animals by allergy skin test. Passive cutaneous anaphylaxis titers of IgG1a-like antibody were zero in control, 1:2 in LD, 1:40 in MD, and 1:160 in HD. Total leukocytes in BALF were increased by CRa challenge in all three CRa-sensitized groups compared with control (p < 0.0001). The tracheal rings from CRa-sensitized guinea pigs constricted upon addition of incremental doses of CRa challenge in vitro in a dose-responsive manner (p < 0.0001). The leukocytosis in BALF and the anaphylactic contractile responses of the tracheal rings in CRa-sensitized groups were correlated to the levels of CRa to which the groups had been exposed during sensitization (p < 0.001). CRa-provoked increase in SRaw was noted in all three groups of the CRa-sensitized guinea pigs compared with control (p < 0.01). Yet, among CRa-sensitized groups, a greater increase in specific airway resistance was noted in the LD group but not in the HD CRa group. Conclusions: The development of anaphylactic cockroach sensitivity in guinea pigs was dependent on the levels of CRa exposure during sensitization, and the CRa-sensitized animals showed antigen-specific airway inflammation along with airway smooth muscle contractions. However, the severity of bronchospasm in conscious animals was not in agreement with the degree of CRa sensitivity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003020413

Download citation: RISBibTeXText

PMID: 9600503

DOI: 10.1016/S0091-6749(98)70174-5

Related references

A dose relationship to cockroach allergen sensitization and asthma in Hartley guinea pigs. Journal of Allergy & Clinical Immunology 99(1 PART 2): S500, 1997

Comparative study on ovalbumin and cockroach allergen induced allergic sensitization and asthmatic responses in Hartley guinea pigs. Journal of Allergy & Clinical Immunology 101(1 PART 2): S200, 1998

Is there a dose-response relationship between exposure to indoor allergens and symptoms of asthma?. Journal of Allergy and Clinical Immunology 96(4): 435-440, 1995

Effects of exposure to sulfuric acid mist on the induction of experimental asthma in guinea pigs: dynamics of histamine in the lung. Arerugi 35(2): 137-144, 1986

Participation of encephalitogen in incomplete Freund's adjuvant in the induction of experimental allergic encephalomyelitis in Hartley guinea pigs. Journal of Neuroimmunology 6(3): 187-196, 1984

The paradoxical relationship between animal dander, exposure, sensitization and asthma: is there a hierarchy of inhalant allergens?. Clinical & Experimental Allergy Reviews 4(Supplement S2): 237-241, 2004

Exposure to cockroach allergens is related with asthma morbidity in cockroach sensitized patients. Allergy (Copenhagen) 53(SUPPL 43): 175, 1998

Sensitization to cockroach allergens of asthma patients in Japan. Arerugi 43(11): 1309-1315, 1994

A comparative study on cockroach and ovalbumin sensitizations and challenge responses in Hartley guinea-pigs. Respiration Physiology 125(3): 239-247, April, 2001

The role of sensitization to cockroach allergens in the etiology of atopic bronchial asthma. Immunologiya 0(6): 45-47, 1996

Sensitization to indoor allergens in patients with cockroach asthma and rhinitis in Belarus. Journal of Allergy & Clinical Immunology 111(2 Abstract Supplement): S160, February, 2003

Effects of exposure to sulfuric acid mist on the induction of experimental asthma in guinea pigs studies on the breathing curve pattern and pulmonary beta adrenergic receptor. Japanese Journal of Allergology 35(6): 402-408, 1986

Prevalence of sensitivity to cockroach allergens and IgE cross-reactivity between cockroach and house dust mite allergens in Chinese patients with allergic rhinitis and asthma. Chinese Medical Journal 123(24): 3540-3544, 2012

Investigating the relationship of current cockroach allergen exposure to cockroach sensitization among Barbadian asthmatics and their family members. Journal of Allergy & Clinical Immunology 107(2): S73, February, 2001

Cockroach allergens, environmental exposure, and asthma. Allergy And Allergic Diseasese 1: The Scientific Basis Of Allergy, 2008