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A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector


A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector



Febs Letters 442(2-3): 183-188



ISSN/ISBN: 0014-5793

PMID: 9928998

DOI: 10.1016/s0014-5793(98)01647-0

Monoclonal antibody 4C1 specifically binds to and neutralizes the most potent neurotoxin (AahII) of the scorpion Androctonus australis. The cDNAs encoding the variable regions of this antibody were isolated by PCR-mediated cloning. A single-chain Fv gene was engineered and expressed in Escherichia coli. The recombinant protein had neutralizing activity similar to that of the intact antibody in vitro and in vivo. We have thus neutralized the pharmacological and biological properties of a scorpion neurotoxin with a single-chain Fv, which opens new perspectives for the treatment of envenomizations.

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Accession: 003025701

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Related references

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