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A simple and efficient protocol for isolation of high molecular weight DNA from the cyanobacterium Spirulina platensis



A simple and efficient protocol for isolation of high molecular weight DNA from the cyanobacterium Spirulina platensis



Biotechnology Techniques 11(9): 631-632



An efficient protocol which involved mechanical destruction of cell walls by freeze thawing and removal of polysaccharide by cetyltrimethylammonium bromide precipitation is described for the rapid isolation of high molecular weight ( gtoreq 50 kb) DNA from the Spirulina platensis. The DNA yield ranged 1.0-1.54 mu-g/mg dry wt with the purity measured at A-260/280 of 1.77-1.82. The DNA preparation was suited as substrate for restriction and PCR.

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