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A study on an appropriate culturing time for in vitro maturation of bovine oocytes


, : A study on an appropriate culturing time for in vitro maturation of bovine oocytes. Kaen Kaset = Khon Kaen Agriculture Journal 25(3): 146-150

Cattle oocytes, collected at a local slaughterhouse, were divided into 3 groups which were cultured for 24, 27 and 30 h. The oocytes were cultured in TCM-199 with 10% fetal calf serum and incubated at 39 degrees C with 5% CO2 and high humidity. The maturation rates (development to metaphase 2) of oocytes cultured for 24, 27 and 30 h were 4.08, 48.00 and 51.79% respectively.

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