+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Accumulation of a clock-regulated transcript during flower-inductive darkness in pharbitis nil

Accumulation of a clock-regulated transcript during flower-inductive darkness in pharbitis nil

Plant Physiology 116(4): 1479-1485

To clarify the molecular basis of the photoperiodic induction of flowering in the short-day plant Pharbitis nil cv Violet, we examined changes in the level of mRNA in cotyledons during the flower-inductive photoperiod using the technique of differential display by the polymerase chain reaction. A transcript that accumulated during the inductive dark period was identified and a cDNA corresponding to the transcript, designated PnC401 (P. nil C401), was isolated. RNA-blot hybridization verified that levels of PnC401 mRNA fluctuated with a circadian rhythm, with maxima between 12 and 16 h after the beginning of the dark period) and minima of approximately 0. This oscillation continued even during an extended dark period but was damped under continuous light. Accumulation of PnC401 mRNA was reduced by a brief exposure to red light at the 8th h of the dark period (night-break treatment) or by exposure to far-red light at the end of the light period (end-of-day far-red treatment). These results suggest that fluctuations in levels of PnC401 mRNA are regulated by phytochrome(s) and a circadian clock and that they are associated with photoperiodic events that include induction of flowering.

(PDF emailed within 0-6 h: $19.90)

Accession: 003030378

Download citation: RISBibTeXText

PMID: 9536066

DOI: 10.1104/pp.116.4.1479

Related references

Changes in in vitro translated polypeptides of Pharbitis nil Chois., strain Violet cotyledons during the first flower-inductive and non-inductive photoperiods. Plant Science Limerick 78(1): 11-18, 1991

In vitro translated polypeptides of different organs of pharbitis nil chois. strain violet under flower inductive and non inductive conditions. Plant Science (Shannon) 58(1): 1-8, 1988

Circadian-regulated accumulation of a transcript encoding a leaf-specific germin-like protein in Pharbitis nil and Cucumis sativus. Plant Physiology (Rockville) 114(3 SUPPL ): 240, 1997

Changes in polypeptides in Pharbitis cotyledons during the first flower-inductive photoperiod. Plant Science (Limerick) 89(2): 135-145, 1993

Photoperiodism and rhythmic flower induction: complete substitution of inductive darkness by light. Canadian journal of botany: 47(8) 1241-1250, 1969

Light- and circadian clock-regulated expression of S-adenosylmethionine decarboxylase gene in Pharbitis nil. Plant Biology (Rockville) : 196, 2000

Role of the photoperiod preceding a flower inductive dark period in dark grown seedlings of pharbitis nil. Plant & Cell Physiology 25(5): 715-720, 1984

The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil. Acta Physiologiae Plantarum 35(3): 801-810, 2013

Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition. Journal of Plant Physiology 166(2): 192-202, 2009

Cyclic GMP stimulates flower induction of Pharbitis nil via its influence on cGMP regulated protein kinase. Plant Growth Regulation 57(2): 115-126, 2009

Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness. Functional & Integrative Genomics 16(4): 399-418, 2016

Varietal difference in the ability to flower in response to poor nutrition and its correlation with chlorogenic acid accumulation in pharbitis nil. Plant & Cell Physiology 29(4): 611-614, 1988

Cryptochrome and Period Proteins Are Regulated by the CLOCK/BMAL1 Gene: Crosstalk between the PPARs/RXRalpha-Regulated and CLOCK/BMAL1-Regulated Systems. Ppar Research 2008: 348610-348610, 2008