+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Adenophostin, a potent agonist of the inositol 1,4,5-trisphosphate receptor, is useful for fertilization of mouse oocytes injected with round spermatids leading to normal offspring

Adenophostin, a potent agonist of the inositol 1,4,5-trisphosphate receptor, is useful for fertilization of mouse oocytes injected with round spermatids leading to normal offspring

Biology of Reproduction 58(3): 867-873

ISSN/ISBN: 0006-3363

PMID: 9510978

DOI: 10.1095/biolreprod58.3.867

Precursor male gametes such as round spermatids and secondary spermatocytes are known to possess the potential to achieve fertilization and embryonic development when injected into mature oocytes, but in previous studies, injected mouse spermatids did not activate the oocytes. In this study, we confirmed that this was the case because spermatids by themselves could not induce an increase in intracellular Ca2+ concentration ([Ca2+]i) of oocytes, the pivotal signal in oocyte activation. Repetitive rises in [Ca2+]i (Ca2+ oscillations), lasting for at least 3 h as observed at fertilization, were produced by a single injection of adenophostin B isolated from fungal products, a novel nonmetabolizable agonist of the inositol 1,4,5-trisphosphate receptor (InsP3r), which mediates Ca2+ release from the endoplasmic reticulum. Ca2+ oscillations were blocked by an antibody against the type 1 InsP3r. About 95% of oocytes were activated by adenophostin (0.3-0.4 microM in the oocyte). Simultaneous injection of a round spermatid and adenophostin resulted in 55% fertilization success in association with male and female pronucleus formation and development to two-cell embryos. Furthermore, 25% of two-cell embryos that were transplanted to foster mothers developed to normal offspring. All infants grew into adults that reproduced healthy second generations. Adenophostin will be useful for parthenogenetic oocyte activation in the biotechnology of animal reproduction. Injection combined with precursor spermatozoa may be applicable to assisted conception therapy for patients with defective spermatogenesis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003032038

Download citation: RISBibTeXText

Related references

Isoforms of the inositol 1,4,5-trisphosphate receptor are expressed in bovine oocytes and ovaries: the type-1 isoform is down-regulated by fertilization and by injection of adenophostin A. Biology of Reproduction 61(4): 935-943, 1999

Inositol Adenophostin: Convergent Synthesis of a Potent Agonist of d- myo -Inositol 1,4,5-Trisphosphate Receptors. Acs Omega 5(44): 28793-28811, 2020

Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring. Journal of Assisted Reproduction and Genetics 13(5): 431-434, 1996

Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development 121(8): 2397-2405, 1995

2-Hydroxyethyl-alpha-D-glucopyranoside-2,3',4'-trisphosphate, a novel, metabolically resistant, adenophostin A and myo-inositol-1,4,5-trisphosphate analogue, potently interacts with the myo-inositol-1,4,5-trisphosphate receptor. Molecular Pharmacology 47(6): 1204-1211, 1995

2-Hydroxyethyl alpha-D-glucopyranoside 2,3',4'-trisphosphate: a novel metabolically resistant adenophostin A and myo-inositol 1,4,5-trisphosphate analogue potently interacts with the myo-inositol 1,4,5-trisphosphate receptor. Biochemical Society Transactions 23(3): 420S, 1995

Molecular recognition of adenophostin, a very potent Ca2+ inducer, at the D-myo-inositol 1,4,5-trisphosphate receptor. Biochemistry 38(29): 9234-9241, 1999

Inositol 1,4,5-trisphosphate receptors are downregulated in mouse oocytes in response to sperm or adenophostin A but not to increases in intracellular Ca(2+) or egg activation. Developmental Biology 223(2): 251-265, 2000

Inositol 1,4,5-trisphosphate receptors are downregulated in mouse oocytes in response to sperm or adenophostin A but not increases in intracellular Ca2+ or oocyte activation. Journal of Reproduction and Fertility Abstract Series (25): 15, 2000

6-Deoxy-6-hydroxymethyl scyllo-inositol 1,2,4-trisphosphate: A potent agonist at the inositol 1,4,5-trisphosphate receptor. Bioorganic and Medicinal Chemistry Letters 6(18): 2197-2200, 1996

Sperm-induced Ca(2+) oscillations in mouse oocytes and eggs can be mimicked by photolysis of caged inositol 1,4,5-trisphosphate: evidence to support a continuous low level production of inositol 1, 4,5-trisphosphate during mammalian fertilization. Developmental Biology 225(1): 1-12, 2000

Sperm-induced Ca2+ oscillations in mouse oocytes can be mimicked precisely by photolysis of caged inositol 1,4,5-trisphosphate Evidence in favour of a continuous low level production of inositol 1,4,5-trisphosphate during mammalian fertilization. Journal of Reproduction and Fertility Abstract Series (25): 15-16, 2000

Synthesis of potent agonists of the D-myo-inositol 1,4, 5-trisphosphate receptor based on clustered disaccharide polyphosphate analogues of adenophostin A. Journal of Medicinal Chemistry 43(17): 3295-3303, 2000

Adenophostin A and inositol 1,4,5-trisphosphate confers distinct calcium dependencies of inositol trisphosphate receptor gating, dependent on ATP. Journal of General Physiology 116(1): 15a, 2000

Development of normal offspring from mouse oocytes injected with mature spermatozoa, testicular spermatozoa, spermatids and secondary spermatocytes. Biology of Reproduction 52(Suppl. 1): 139, 1995