EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Alterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni


, : Alterations in cytochrome-c oxidase expression between praziquantel-resistant and susceptible strains of Schistosoma mansoni. Parasitology 117: 63-73

The genetic differences between praziquantel-resistant (R) and susceptible (S) strains of Schistosoma mansoni (Fallon & Doenhoff, 1994) were explored using RAPD and by cloning differentially expressed mRNAs by subtractive PCR. No differences between the 2 strains were detectable by RAPD using 41 different primers indicating that no major genomic rearrangements were present. Subtractive PCR generated a number of fragments, 1 of which was shown to correspond to an over-expressed mRNA in the R strain and to encode a fragment of the subunit 1 of cytochrome-c oxidase (SCOX1). In the absence of a complete sequence for this gene, we used EST sequences to compile a consensus sequence for the 904 bp at the 3' end that enabled us to choose primers for semi-quantitative RT-PCR. This technique showed that SCOX1 was indeed over-expressed about 5 to 10-fold in the R strain whereas the genes encoding the 28 kDa glutathione S-transferase, glutathione peroxidase, NADH dehydrogenase subunit 5 and the ATP-binding cassette family protein SMDR2 were not. In contrast, cytochrome-c oxidase enzyme activity was 4-fold lower in the R strain than in the S strain.


Accession: 003035756

PMID: 9695101

DOI: 10.1017/s003118209800273x

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Anonymous, 2007: Studies on resistance of Schistosoma to praziquantel XII. Comparison of susceptibility between male and female cercariae of praziquantel resistant and susceptible isolates of Schistosoma mansoni to praziquantel

Liang YouSheng; Dai JianRong; Zhu YinChang; X.M.ng; X.Y.ngLiang; Hang PanYu; L.H.ngJun; Coles, G.C.; Doenhoff, M.J., 2002: Studies on resistance of Schistosoma to praziquantel. II. Responses of eggs and miracidia of praziquantel-resistant and -susceptible Schistosoma mansoni to praziquantel. Objective: To develop a test in vitro to detect the susceptibility of Schistosoma mansoni to praziquantel and monitor for the presence of praziquantel-resistance in the field, by comparing the responses of eggs and miracidia of praziquantel-resist...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2003: Studies on resistance of Schistosoma to praziquantel. IV. Response of adults of praziquantel-resistant and -susceptible Schistosoma mansoni to praziquantel in vitro. Mature adults of various isolates of Schistosoma mansoni were incubated at 37 degrees C in MEM medium containing 3.2x10-4, 8x10-4 and 1.6x10-3 mol/litre praziquantel. The survival status of S. mansoni were observed on the basis of body contractili...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2003: Studies on resistance of Schistosoma to praziquantel III. Response of cercariae of praziquantel-resistant and -susceptible Schistosoma mansoni to praziquantel. The presence of praziquantel-resistant Schistosoma mansoni in snails (Biomphalaria glabrata) collected from the field was monitored by comparing the responses of praziquantel-resistant and -susceptible S. mansoni to 10-4, 10-5, 6x10-7 and 4x10-7 m...

Pinto-Almeida, Aónio.; Mendes, T.; de Oliveira, R.Nunes.; Corrêa, S.de.Andrade.Penteado.; Allegretti, S.Marques.; Belo, S.; Tomás, A.; Anibal, F.de.Freitas.; Carrilho, E.; Afonso, A., 2016: Morphological Characteristics of Schistosoma mansoni PZQ-Resistant and -Susceptible Strains Are Different in Presence of Praziquantel. Schistosomiasis is one of the most common human parasitic diseases whose socioeconomic impact is only surpassed by malaria. Praziquantel (PZQ) is the only drug commercially available for the treatment of all schistosome species causing disease in...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; S.J.n; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2004: Studies on resistance of Schistosoma to praziquantel. VIII. Infectivity and egg productions of praziquantel-resistant and -susceptible isolates of Schistosoma mansoni in mice. Objective: To investigate the change in the pathogenicity of Schistosoma mansoni associated with the development of resistance to praziquantel in definitive hosts. Methods: Outbred CD1 albino mice were infected with either 5 praziquantel-resistant...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; S.J.n; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2003: Studies on resistance of Schistosoma to praziquantel VII. Some biological characteristics of praziquantel-resistant and -susceptible isolates of Schistosoma mansoni. The fecundity of four praziquantel (PZQ)-resistant and three PZQ-susceptible isolates of S. mansoni was compared in Biomphalaria glabrata snails and outbred CD1 albino mice. When snails were exposed to a single miracidium per snail for 2 h, the in...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; S.J.n; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2003: Studies on resistance of Schistosoma to praziquantel: VI. Effect on formation of egg-granulomas in livers of praziquantel-resistant and -susceptible Schistosoma mansoni treated in vivo. A study was conducted to compare the effect of praziquantel on the formation of egg-granulomas in the livers of mice infected with Schistosoma mansoni worms (praziquantel-resistant and -susceptible parasite isolates). Two praziquantel-resistant is...

Liang YouSheng; Dai JianRong; Zhu YinChang; L.H.ngJun; X.M.ng; S.J.n; X.Y.ngLiang; Hang PanYu; Coles, G.C.; Doenhoff, M.J., 2003: Studies on resistance of Schistosoma to praziquantel: V. Adult worm tegumental damage in praziquantel-resistant and -susceptible Schistosoma mansoni treated in vivo. Objective: To compare the effects of praziquantel treatment on the tegument of praziquantel-resistant and -susceptible adult Schistosoma mansoni isolates in vivo. Methods: Two groups of mice were infected with either of the 'gene-pooled'...

Liang YouSheng; Coles, G.C.; Doenhoff, M.J.; Southgate, V.R., 2001: In vitro responses of praziquantel-resistant and -susceptible Schistosoma mansoni to praziquantel. The resistance status of five praziquantel-susceptible and five praziquantel-resistant isolates was confirmed by chemotherapy in CD(1) mice with 3 x 200mg/kg micronised praziquantel. Micronised praziquantel had higher efficacy than two other prazi...