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Alterations in nptII and gus expression following micropropagation of transgenic M.7 apple rootstock lines


, : Alterations in nptII and gus expression following micropropagation of transgenic M.7 apple rootstock lines. Journal of the American Society for Horticultural Science 123(1): 11-18

Seven nptII and gus transgenic lines of apple (Malus domestica rootstock Malling 7 (M.7), produced via Agrobacterium tumefaciens-mediated transformation, were examined by beta -glucuronidase (GUS) histochemical testing and DAS-ELISA. These lines expressed different amounts of neomycin phosphotransferase II (NPTII). NPTII expression among lines was positively correlated with the ability of the transgenic lines to regenerate in the presence of kanamycin, paromomycin or geneticin.


Accession: 003035771

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