EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

An alternative detection method for labeled DNA probes in porcine gene mapping by in situ hybridization



An alternative detection method for labeled DNA probes in porcine gene mapping by in situ hybridization



Allattenyesztes es Takarmanyozas 48(1): 47-50, 158



A method of non-radioactive, non-fluorescence in situ hybridization is described. It is based on the detection of a hybridized, biotinylated probe by peroxidase-conjugated extravidin, and the reaction of peroxidase with diaminobenzidine substrate, resulting in the formation of a dark precipitate.

(PDF 0-2 workdays service: $29.90)

Accession: 003037052

Download citation: RISBibTeXText



Related references

Gene mapping on human metaphase chromosomes by in situ hybridization with 3H, 35S, and 32P labeled probes and transmission electron microscopy. Chromosoma 93(4): 305-312, 1986

In situ hybridization with digoxigenin-labeled probes: Sensitive and reliable detection method applied to myelinating rat brain. Acta Neuropathologica 84(6): 581-587, 1992

Gene mapping on human metaphase chromosomes by in situ hybridization with tritium sulfur 35 and phosphorus 32 labeled probes and transmission electron microscopy. Chromosoma (Berlin) 93(4): 305-312, 1986

In situ hybridization ish detection of rrna rdna in giardia trophozoites and cysts using oligomeric probes directly conjugated with carboxymethylindocyanine dyes or indirect detection with digoxygenin labeled probes use of cytoskeletal preparations to facilitate nuclear detection of dna. Journal of Cell Biology 115(3 PART 2): 87A, 1991

Detection of human papillomavirus type 60 in plantar cysts and verruca plantaris by the in situ hybridization method using digoxigenin labeled probes. Journal of Dermatology 21(10): 709-715, 1994

Fluorescence in situ hybridization on metaphase chromosomes with biotinylated probes. In situ hybridization, biotin labeling, cosmids, gene mapping, oncogene amplification. Nouvelle Revue Francaise D'hematologie 32(6): 459-460, 1990

In situ hybridization with digoxigenin labeled oligonucleotide probes: detection of CAMK-II gene expression in primary cultures of cerebral endothelial cells. Neurobiology 1(3): 235-240, 1993

Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections. Modern Pathology 4(1): 81-85, 1991

Comparison of in situ dna hybridization protocols using sulfur 35 labeled and biotin labeled probes in detection of human papillomavirus dna sequences. Steinberg, B M , J L Brandsma And L B Taichman (Ed ) Cancer Cells (Cold Spring Harbor), 5 Papillomaviruses; Meeting, Cold Spring Harbor, New York, Usa, 1986 Xxvii+423p Cold Spring Harbor Laboratory: Cold Spring Harbor, New York, Usa Illus Paper 329-336, 1987

Mapping muscle protein genes by in situ hybridization using biotin-labeled probes. Embo Journal 4(10): 2493-2498, 1985

Autoradiographic visualization of 35S-labeled cRNA probes combined with immunoperoxidase detection of choleragenoid: a double-labeling light microscopic method for in situ hybridization and retrograde tract tracing. Journal of Histochemistry and Cytochemistry 42(6): 827-831, 1994

Use of digoxigenin-labeled probes for the detection of papillomaviruses by in situ hybridization. Annales de Pathologie 10(5-6): 351-354, 1990

Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes. American Journal of Pathology 131(1): 1-4, 1988

Chromogenic in situ hybridization A new practical alternative to Fluorescent in situ hybridization for detection of HER-2/neu gene amplification in human breast carcinomas. Proceedings of the American Association for Cancer Research Annual Meeting 44: 498, July, 2003