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An alternative detection method for labeled DNA probes in porcine gene mapping by in situ hybridization


, : An alternative detection method for labeled DNA probes in porcine gene mapping by in situ hybridization. Allattenyesztes es Takarmanyozas 48(1): 47-50, 158

A method of non-radioactive, non-fluorescence in situ hybridization is described. It is based on the detection of a hybridized, biotinylated probe by peroxidase-conjugated extravidin, and the reaction of peroxidase with diaminobenzidine substrate, resulting in the formation of a dark precipitate.

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