+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase

An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase

Journal of Agricultural & Food Chemistry 45(8): 2984-2988

Horseradish peroxidase (HRP, EC catalyzed oxidation of betanine is complex, as judged by the shape of the consecutive absorption spectra of the reaction medium. Graphic analysis of these spectra by a matricial test revealed the presence of at least three kinetically related absorbent species in the reaction mixture. Analysis by HPLC confirmed the presence of several reaction products. During the course of the reaction, a red intermediate and several yellow products, presumably of a polymeric nature, are generated, betalamic acid being one of the final products. Even in the absence of enzyme, the reaction, once initiated, continued again, yielding betalamic acid as a final product, which is formed as both substrate and intermediate product disappear. The chemical nature of the intermediate product and the possible reaction mechanism are discussed.

Accession: 003037455

Download citation: RISBibTeXText

DOI: 10.1021/jf970187m

Download PDF Full Text: An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase

Related references

Mechanism of nadph oxidation catalyzed by horseradish peroxidase and 2 4 diacetyl deuterated heme substituted horseradish peroxidase. European Journal of Biochemistry 201(2): 507-514, 1991

Irreversible binding of quinacrine to nucleic acids during horseradish peroxidase catalyzed oxidation and prostaglandin synthetase catalyzed oxidation. Biochemical Pharmacology 32(17): 2604-2607, 1983

Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol. Analytical Biochemistry 240(1): 119-125, 1996

Horseradish peroxidase-catalyzed oxidation of chlorophyll a with hydrogen peroxide: characterization of the products and mechanism of the reaction. Biochimica et Biophysica Acta 1797(5): 531-542, 2010

Oxidation of 2,4-dichlorophenol catalyzed by horseradish peroxidase: Characterization of the reaction mechanism by UV-visible spectroscopy and mass spectrometry. Journal of Inorganic Biochemistry 95(2-3): 171-176, 1 June, 2003

Super oxide and peroxidase catalyzed reactions oxidation of di hydroxy fumarate nadh and di thio threitol by horseradish peroxidase. Photochemistry & Photobiology 28(4/5): 757-763, 1978

Horseradish peroxidase-catalyzed oxidation of rifampicin: reaction rate enhancement by co-oxidation with anti-inflammatory drugs. Biological & Pharmaceutical Bulletin 28(10): 1822-1826, 2005

Horseradish peroxidase catalyzed oxidation of thiocyanate by hydrogen peroxide: comparison with lactoperoxidase-catalysed oxidation and role of distal histidine. Biochimica et Biophysica Acta 1080(1): 45-50, 1991

Mechanism of horseradish peroxidase-catalyzed oxidation of malonaldehyde. Archives of Biochemistry and Biophysics 272(1): 185-193, 1989

Cysteine and crocin oxidation catalyzed by horseradish peroxidase. Free Radical Research Communications 5(4-5): 259-265, 1989

Horseradish peroxidase catalyzed oxidation of mitoxantrone spectrophotometric and epr studies. Journal of Free Radicals in Biology & Medicine 2(1): 25-32, 1986

2,3-Diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine. Analytical Biochemistry 165(1): 230-233, 1987

Human myeloperoxidase and horseradish peroxidase catalyzed oxidation of phenol. Federation Proceedings 45(3): 640, 1986

Horseradish d peroxidase catalyzed iodide oxidation and tyrosine iodination. Scandinavian Journal of Clinical & Laboratory Investigation Supplement 27(116):, 1971