EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase






Journal of Agricultural & Food Chemistry 45(8): 2984-2988

An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase

Horseradish peroxidase (HRP, EC 1.1.11.7) catalyzed oxidation of betanine is complex, as judged by the shape of the consecutive absorption spectra of the reaction medium. Graphic analysis of these spectra by a matricial test revealed the presence of at least three kinetically related absorbent species in the reaction mixture. Analysis by HPLC confirmed the presence of several reaction products. During the course of the reaction, a red intermediate and several yellow products, presumably of a polymeric nature, are generated, betalamic acid being one of the final products. Even in the absence of enzyme, the reaction, once initiated, continued again, yielding betalamic acid as a final product, which is formed as both substrate and intermediate product disappear. The chemical nature of the intermediate product and the possible reaction mechanism are discussed.

Accession: 003037455

DOI: 10.1021/jf970187m

Download PDF Full Text: An approach to the characterization of betanine oxidation catalyzed by horseradish peroxidase



Related references

D.S.ndro V.; Dupuy C.; Kaniewski J.; Ohayon R.; Deme D.; Virion A.; Pommier J., 1991: Mechanism of nadph oxidation catalyzed by horseradish peroxidase and 2 4 diacetyl deuterated heme substituted horseradish peroxidase. The mechanism of NADPH oxidation catalyzed by horse-radish peroxidase (HRP) and 2,4-diacetyl-[2H]heme-substituted peroxidase (DHRP) was studied. The roles of different H2O2/peroxidase compounds were examined by spectral studies. The oxidized NADPH...

Sinha B.K., 1983: Irreversible binding of quinacrine to nucleic acids during horseradish peroxidase catalyzed oxidation and prostaglandin synthetase catalyzed oxidation. Biochemical Pharmacology 32(17): 2604-2607

Kricka, L.J.; Cooper, M.; Ji, X., 1996: Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol. 4-Iodophenylboronic acid has been synthesized and shown to be a potent enhancer of the chemiluminescent horseradish peroxidase (Type VI-A)-catalyzed oxidation of luminol and isoluminol. The enhancer was effective (>100-fold enhancement) in the...

Hynninen, P.H.; Kaartinen, V.; Kolehmainen, E., 2010: Horseradish peroxidase-catalyzed oxidation of chlorophyll a with hydrogen peroxide: characterization of the products and mechanism of the reaction. Horseradish peroxidase was verified to catalyze, without any phenol, the hydrogen peroxide oxidation of chlorophyll a (Chl a), solubilized with Triton X-100. The 13(2)(S) and 13(2)(R) diastereomers of 13(2)-hydroxyChl a were characterized as major...

Laurenti, E.; Ghibaudi, E.; Ardissone, S.; Ferrari, R.Pia., 2003: Oxidation of 2,4-dichlorophenol catalyzed by horseradish peroxidase: characterization of the reaction mechanism by UV-visible spectroscopy and mass spectrometry. The hydrogen peroxide-oxidation of 2,4-dichlorophenol catalyzed by horseradish peroxidase has been studied by means of UV-visible spectroscopy and mass spectrometry in order to clarify the reaction mechanism. The dimerization of 2,4-dichlorophenol...

Laurenti, E.; Ghibaudi, E.; Ardissone, S.; Ferrari, R.P.a, 2003: Oxidation of 2,4-dichlorophenol catalyzed by horseradish peroxidase: Characterization of the reaction mechanism by UV-visible spectroscopy and mass spectrometry. The hydrogen peroxide-oxidation of 2,4-dichlorophenol catalyzed by horseradish peroxidase has been studied by means of UV-visible spectroscopy and mass spectrometry in order to clarify the reaction mechanism. The dimerization of 2,4-dichlorophenol...

Halliwell B.; D.R.cker J., 1978: Super oxide and peroxidase catalyzed reactions oxidation of di hydroxy fumarate nadh and di thio threitol by horseradish peroxidase. Photochemistry & Photobiology 28(4/5): 757-763

dos Santos, F.de.Jesus.Notário.; Ximenes, V.Farias.; da Fonseca, L.Marcos.; de Faria Oliveira, O.Maria.Mascarenhas.; Brunetti, I.Lourenço., 2005: Horseradish peroxidase-catalyzed oxidation of rifampicin: reaction rate enhancement by co-oxidation with anti-inflammatory drugs. The tuberculostatic drug rifampicin has been described as a scavenger of reactive species. Additionally, the recent demonstration that oral therapy with a complex of rifampicin and horseradish peroxidase (HRP) was more effective than rifampicin al...

Modi, S.; Behere, D.V.; Mitra, S., 1991: Horseradish peroxidase catalyzed oxidation of thiocyanate by hydrogen peroxide: comparison with lactoperoxidase-catalysed oxidation and role of distal histidine. Horseradish peroxidase-catalysed oxidation of thiocyanate by hydrogen peroxide has been studied by 15N-NMR and optical spectroscopy at different concentrations of thiocyanate and hydrogen peroxide and at different pH values. The extent of the oxid...

MacDonald, I.D.; Dunford, H.B., 1989: Mechanism of horseradish peroxidase-catalyzed oxidation of malonaldehyde. The mechanism of malonaldehyde oxidation by horseradish peroxidase in the presence of manganese(II) and acetate was investigated. Our results show that an apparent oxygenase behavior demonstrated by peroxidase in this system can be explained in te...