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An attempt to use polymerase chain reaction for the detection of Toxoplasma gondii and Neospora caninum


, : An attempt to use polymerase chain reaction for the detection of Toxoplasma gondii and Neospora caninum. Bangladesh Veterinary Journal 30(1/2): 27-33

The PCR has been established as highly sensitive and specific for identification of the Toxoplasma genome (B1 gene). This work aimed to improve DNA purification methods from T. gondii tachyzoites and tissue specimens for amplification. No difference was observed in the results of electrophoresis of the extracted DNA samples with or without RNAase in tachyzoite samples.

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Related references

Ellis, J.T., 1998: Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii. This review summarizes existing knowledge on the development and use of the polymerase chain reaction for the detection of DNA from Neospora and Toxoplasma. Several strategies which utilise the polymerase chain reaction for the diagnosis of toxopl...

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