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An efficient in vitro regeneration protocol for Tagetes minuta






Plant cell tissue and organ culture99 55(3): 211-215

An efficient in vitro regeneration protocol for Tagetes minuta

Tagetes minuta is a source of secondary products which are used as pharmaceuticals, pesticides and as flavour components in the food industry. Cotyledons and hypocotyls of T. minuta were cultured on MS medium with combinations of IAA or NAA and BA. Hypocotyl-derived callus developed adventitious shoots which failed to develop further. Cotyledon-derived callus, cultured on medium with IAA, regenerated adventitious shoots which developed into plantlets on MS medium or half-strength MS with 2.85 micromolar IAA. Cotyledons cultured on medium with 5.71 micromolar IAA + 44.4 micromolar BA and transferred to MS medium for shoot growth yielded the highest number of shoots. Nodal segments from developing shoots were micropropagated on half-strength MS medium with 2.58 micromolar IAA and 95% of plantlets produced adapted successfully to greenhouse conditions. In vitro plants micropropagated from nodes had many shoots whereas plants regenerated from shoot tips had only a single main stem. This difference in morphology was retained after two months growth in a greenhouse. There were no significant differences in leaf and shoot fresh and dry weights among the regenerated plants after two months growth. After six subcultures of cotyledon-derived callus on medium with IAA and BA all explants lost their ability to regenerate except those cultured on medium with 17.23 micromolar IAA and 44.4 micromolar BA. The methods of regeneration developed will facilitate selection of T. minuta plants more tolerant of environmental stress, their micropropagation, and the in vitro production of secondary products.

Accession: 003037872

DOI: 10.1023/a:1004552531347

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Related references

Mohamed, M.A.H.; Harris, P.J.C.; Henderson, J., 1999: An efficient in vitro regeneration protocol for Tagetes minuta. Tagetes minuta is a source of secondary products which are used as pharmaceuticals, pesticides and as flavour components in the food industry. Cotyledons and hypocotyls of T. minuta were cultured on MS medium with combinations of IAA or NAA and BA...

Mohamed, M.A.H.; Harris, P.J.C.; Henderson, J., 1998: In vitro regeneration of Tagetes minuta and the morphology of regenerated plants. Journal of Experimental Botany 49(SUPPL ): 17-18, May

Sharief, M.U., 2004: Induction of somatic embryogenesis and plant regeneration from leaf callus cultures of Tagetes minuta. Somatic embryogenesis was initiated from the leaf segments of Tagetes minuta (commonly known as Stinking Roger) and the cultures were maintained for a period of 2 years on Murashige and Skoog (MS) medium supplemented with NAA at 2 mg litre-1 and B...

Hethelyi E.; Danos B.; Tetenyi P.; Koczka I., 1987: Gc ms analysis of the essential oils of four tagetes species and the microbial activity of tagetes minuta. The chemical composition of the essential oils of the Tagetes species was determined by GC/MS method. The characteristic components of the essential oils produced from the flowering plants of the T. minuta L. and T. tenuifolia Cav. were the .beta....

Anonymous, 1987: Tagetes minuta l synonym tagetes glandulifera fatty acid composition of the seed oil

Hethelyi, E.; Danos, B.; Tetenyi, P.; Koczka, I., 1986: GC-MS analysis of the essential oils of four Tagetes species and the anti-microbial activity of Tagetes minuta. The essential oils were examined by GC-MS. Only T. minuta was rich in beta -ocimene. The other major constituents of this species and of T. tenuifolia were dihydrotagetone, tagetone and (E)-ocimenone. Both species also contained small amounts of (...

Graven, E.H.; Webber, L.; Benians, G.; Venter, M.; Gardner, J.B., 1991: Effect of soil type and nutrient status on the yield and composition of tagetes oil (Tagetes minuta L.). A glasshouse fertilizer trial (omission principle) was conducted to investigate the effect of the nutrient status of 3 problem Ciskei soils on the yield and quality of the essential oil of T. minuta. Acute inherent deficiencies of N and P (3 soils...

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Mohamed, M.A.H.; Harris, P.J.C.; Henderson, J., 2000: In vitro selection and characterisation of a drought tolerant clone of Tagetes minuta. Aromatic Tagetes plants produce secondary products which have a biological activity against a wide range of micro-organisms, insects and nematodes. Tagetes oils are also used as pharmaceuticals and as flavour components in the food industry. This...