EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

An improved procedure for the mass isolation of viable sperm cells from pollen of Zea mays L



An improved procedure for the mass isolation of viable sperm cells from pollen of Zea mays L



Acta Phytophysiologica Sinica 23(3): 245-250



Mass isolation of viable sperm cells from maize pollen was further studied to increase the yield of sperm cells. Four different approaches on pollen bursting and sperm collection were tried. Results showed that pollen bursting rate could reach as high as 95% with BKS35 preincubation followed by buffered osmotic shock solution (in sucrose 0.44 mol/L, Mes 2 mmol/L and 0.1% BSA, pH 6.7). After examining the isolation efficiency of 4 different Percoll solutions, it is found that the higher sperm yield (ca. 23.4%) could be obtained using 30% Percoll in buffered sucrose solution. Using the above modified two-step osmotic shock followed by 30% Percoll centrifugation, about 1.2 X 108 viable sperm cells could be obtained from 100 g fresh pollen sample within 6 h.

(PDF 0-2 workdays service: $29.90)

Accession: 003038598

Download citation: RISBibTeXText



Related references

Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains. Plant Physiology 85(4): 876-878, 1987

Isolation of viable sperm cells from corn zea mays pollen grains. Negrutiu, I And G B Gharti-Chhetri (Ed ) Biomethods, Vol 4 A Laboratory Guide For Cellular And Molecular Plant Biology 386p Birkhaeuser Verlag: Basel, Switzerland; Boston, Massachusetts, Usa Illus 88-96, 1991

Mass isolation of viable sperm cells from the pollen of Liriodendron chinense. Journal of Nanjing Forestry University 23(3): 21-24, 1999

Isolation and characterization of viable sperm cells from tricellular pollen grains. Cresti, M , P Gori And E Pacini (Ed ) Sexual Reproduction in Higher Plants; Tenth International Symposium, Siena, Italy, May 30-June 4, Xiii+502p Springer-Verlag New York, Inc : Secaucus, New Jersey, Usa; Berlin, West Germany Illus 245-250, 1988

Isolation and collection of two populations of viable sperm cells from the pollen of Plumbago zeylanica. Zygote 6(4): 295-298, 1999

Mass isolation of viable sperm cells from rice. Sichuan Daxue Xuebao (Ziran Kexueban) 35(6): 942-945, 1998

Isolation and purification of viable sperm cells from stored bicellular pollen of Lilium davidii Duch. Acta Botanica Sinica 37(8): 589-593, 1995

Isolation and viability of sperm cells from corn (Zea mays) and kale (Brassica oleracea) pollen grains. Plant sperm cells as tools for biotechnology: 105-109, 1988

Mass isolation and preservation of viable sperm cells in brassica campestris var purpurea. Acta Botanica Sinica 33(9): 649-657, 1991

Pollen quality in Zea mays as a prerequisite for sperm cell isolation and pollen transformation. Plant sperm cells as tools for biotechnology: 97-104, 1988

Isolation of two populations of sperm cells and microelectrophoresis of pairs of sperm cells from pollen tubes of tobacco (Nicotiana tabacum). Sexual Plant Reproduction 18(2): 47-53, 2005

Labeling the plasma membrane of isolated viable sperm cells of Zea mays L. by fluorophore-conjugated lectins. Acta Phytophysiologica Sinica 23(4): 399-404, 1997

An improved procedure for isolation of ragweed pollen allergen Ra5. Journal of Immunology 115(1): 303-304, 1975

RNA and protein synthesis in sperm cells isolated from Zea mays L. pollen. Sexual Plant Reproduction 6(4): 239-243, 1993

Structure and properties of sperm cells isolated from the pollen of zea mays. Canadian Journal of Botany 66(5): 819-825, 1988