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Applicability of nine microsatellite DNA sequences vs eleven polymorphic blood protein and enzyme systems for the parentage control in Polish Arabian and Thoroughbred horse



Applicability of nine microsatellite DNA sequences vs eleven polymorphic blood protein and enzyme systems for the parentage control in Polish Arabian and Thoroughbred horse



Animal Science Papers & Reports 16(4): 209-218



The polymorphism of nine microsatellite DNA sequences and 11 blood protein and enzyme systems was studied in 202 Arabian and 98 Thoroughbred horses. Depending on the locus the coefficient of heterozygosity ranged from 0.096 to 0.77 for microsatellite sequences and from 0.029 to 0.73 for blood proteins and enzymes, in Arabian and Thoroughbred horses, respectively. The polymorphic information content was found similar to the coefficient of heterozygosity within individual loci. Basing on the polymorphism of six microsatellite loci in Arabian horses the combined probability of excluding a wrong parent reached 0.778 when the genotype of one parent was known (PE1), and 0.941 when the genotypes of both parents were known (PE2). Within Arabian horses the exclusion probability as estimated on the basis on 11 polymorphic proteins and enzyme systems reached PE1 = 0.685 and PE2 = 0.888. In Thoroughbreds the respective values based on the analysis or seven microsatellites were PE1 = 0.751 and PE2 = 0.932, while PE1 = 0.551 and PE2 = 0.814 as obtained from an analysis of blood protein and enzyme systems polymorphism.

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Accession: 003044646

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