+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Are genotypes of Trypanosoma cruzi involved in the challenge of chagasic cardiomyopathy?

, : Are genotypes of Trypanosoma cruzi involved in the challenge of chagasic cardiomyopathy?. Parasitology Research 84(2): 147-152

Myocardial damage in Chagas' disease differs, depending on the particular Trypanosoma cruzi stock. It is reasonable to expect that the extent of phylogenetic divergence between lineages will have an impact on the biological properties of the parasite. The aim of the present work was to evaluate this impact on the cardiac damage produced by this protozoan. Heart histopathologic lesions were studied in mice infected with 15 cloned stocks of T. cruzi of various origins pertaining to 3 major clones or genotypes (19, 20, and 39) that share 3 different profiles for a given set of genetic markers. Sets of mice were infected intraperitoneally with 10(6) blood trypomastigotes of each of the T. cruzi clones. The macroscopy study showed a cardiac index (CI) higher than 0.6 (cardiomegaly) in 5 of the 15 stocks studied (33.33%). Inflammatory infiltrates appeared in stocks pertaining to the three genotypes studied without relation to the genetic pattern. Pseudocysts were present at higher levels (83%) in stocks pertaining to genotype 39. A lower rate could be seen in stocks pertaining to genotype 19 (50%). Only one stock pertaining to genotype 20 presented myocardial parasites (20%). Hearts were also studied for lesions in the different cardiac chambers. Inflammatory foci as well as pseudocysts appeared mainly in ventricles, with the left ventricle sharing the highest percentage of pathologic findings. In summary, in spite of the similar inflammatory pattern demonstrated for all stocks studied, the parasite's presence seemed to be related to the genotype of reference, but no relation could be demonstrated with the genetic distances.

Accession: 003046135

PMID: 9493216

DOI: 10.1007/s004360050373

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Ramírez, J.David.; Guhl, F.; Rendón, L.María.; Rosas, F.; Marin-Neto, J.A.; Morillo, C.A., 2011: Chagas cardiomyopathy manifestations and Trypanosoma cruzi genotypes circulating in chronic Chagasic patients. Chagas disease caused by Trypanosoma cruzi is a complex disease that is endemic and an important problem in public health in Latin America. The T. cruzi parasite is classified into six discrete taxonomic units (DTUs) based on the recently proposed...

Anonymous, 2012: Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy. The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood. We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms from the...

Solari, A.; Campillay, R.; Ortíz, S.; Wallace, A., 2001: Identification of Trypanosoma cruzi genotypes circulating in Chilean chagasic patients. A study was conducted to evaluate the presence of the most prevalent T. cruzi genotypes circulating in patients with Chagas' disease in 2 geographic regions of Chile, using appropriate probes. Parasite DNA amplified using polymerase chain rea...

O'Daly, J.A.; Carrasco, H.; Fernandez, V.; Rodríguez, M.B., 1994: Comparison of chagasic and non-chagasic myocardiopathies by ELISA and immunoblotting with antigens of Trypanosoma cruzi and Trypanosoma rangeli. Trypanosoma cruzi associated myocardiopathy, or Chagas disease, continues to be a serious problem in Venezuela, for which there is neither a vaccine nor a cure. In order to learn more about the humoral immune response to trypanosomal antigens, and...

Jones E.M.; Colley D.G.; Tostes S.Jr; Lopes E.R.; Vnencak Jones C.L.; Mccurley T.L., 1992: Amplification of a trypanosoma cruzi dna sequence from inflammatory lesions of human chagasic cardiomyopathy. Clinical Research 40(2): 330A

Jones, E.M.; Colley, D.G.; Tostes, S.; Lopes, E.R.; Vnencak-Jones, C.L.; McCurley, T.L., 1993: Amplification of a Trypanosoma cruzi DNA sequence from inflammatory lesions in human chagasic cardiomyopathy. The major cause of morbidity and mortality in Chagas' disease is a chronic inflammatory cardiomyopathy, which presents ten or more years following initial infection. Demonstration of Trypanosoma cruzi in cardiac tissue by routine microscopy o...

Jones, E.M.; Colley, D.G.; Tostes, S.; Lopes, E.R.; Vnencak-Jones, C.L.; McCurley, T.L., 1992: A Trypanosoma cruzi DNA sequence amplified from inflammatory lesions in human chagasic cardiomyopathy. Transactions of the Association of American Physicians 105: 182-189

Levin, M.J., 1999: Contribution of the Trypanosoma cruzi Genome Project to the understanding of the pathogenesis of chronic chagasic cardiomyopathy. The Trypanosoma cruzi Genome Project (TcGP) provides researchers and clinicians with information on gene expression in T. cruzi (see TcGP database, and with a considerable number of genomic libraries and probes (expres...

Leon Rodriguez, D.A.; González, C.I.; Martin, J., 2016: Analysis of association of FOXO3 gene with Trypanosoma cruzi infection and chronic Chagasic cardiomyopathy. FOXO3, a member of the Forkhead family of proteins, plays a role in controlling immune response. FOXO3 gene variant rs12212067 has been associated to differential severity of infectious diseases like malaria. In this study, we assessed whether thi...

Garzón, E.A.; Barnabé, C.; Córdova, X.; Bowen, C.; Paredes, W.; Gómez, E.; Ouaissi, A.; Tibayrenc, M.; Guevara, A.G., 2002: Trypanosoma cruzi isoenzyme variability in Ecuador: first observation of zymodeme III genotypes in chronic chagasic patients. We have analysed by multilocus enzyme electrophoresis (MLEE) at 21 genetic loci 10 Trypanosoma cruzi stocks isolated from chronic chagasic patients and 3 stocks isolated from Triatoma dimidiata collected in human habitats from the coastal part of...