EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages



Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages



Applied and Environmental Microbiology 63(11): 4485-4493



A Burkholderia cepacia population naturally occurring in the rhizosphere of Zea mays was investigated in order to assess the degree of root association and microbial biodiversity at five stages of plant growth. The bacterial strains isolated on semiselective PCAT medium were mostly assigned to the species B. cepacia by an analysis of the restriction patterns produced by amplified DNA coding for 16S rRNA (16S rDNA) (ARDRA) with the enzyme AluI. Partial 16S rDNA nucleotide sequences of some randomly chosen isolates confirmed the ARDRA results. Throughout the study, B. cepacia was strictly associated with maize roots, ranging from 0.6 to 3.6% of the total cultivable microflora. Biodiversity among 83 B. cepacia isolates was analyzed by the random amplified polymorphic DNA (RAPD) technique with two 10-mer primers. An analysis of RAPD patterns by the analysis of molecular variance method revealed a high level of intraspecific genetic diversity in this B. cepacia population. Moreover, the genetic diversity was related to divergences among maize root samplings, with microbial genetic variability markedly higher in the first stages of plant growth; in other words, the biodiversity of this rhizosphere bacterial population decreased over time.

(PDF 0-2 workdays service: $29.90)

Accession: 003052960

Download citation: RISBibTeXText

PMID: 9361434



Related references

Frequency and biodiversity of 2,4-diacetylphloroglucinol-producing bacteria isolated from the maize rhizosphere at different stages of plant growth. Applied and Environmental Microbiology 66(3): 948-955, 2000

Characterization of a free-living maize-rhizosphere population of Burkholderia cepacia: effect of seed treatment on disease suppression and growth promotion of maize. FEMS Microbiology Ecology 27(3): 225-237, 1998

Maize rhizosphere in Sichuan, China, hosts plant growth promoting Burkholderia cepacia with phosphate solubilizing and antifungal abilities. Microbiological Research 169(1): 76-82, 2014

Biological control of Fusarium spp. by a maize rhizosphere population of Burkholderia cepacia. Bulletin OILB/SROP 21(9): 283-289, 1998

Genetic relationships among Italian and Mexican maize-rhizosphere Burkholderia cepacia complex (BCC) populations belonging to Burkholderia cenocepacia IIIB and BCC6 group. Bmc Microbiology 11(): 228-228, 2012

Factors affecting the competitive ability in rhizosphere colonization of plant-growth promoting strains of Burkholderia cepacia. Ryder, M H, Stephens, P M, Bowen, G D Improving plant productivity with rhizosphere bacteria 204-206, 1994

Burkholderia cepacia, a plant growth promoting rhizobacterial associate of maize. Ryder, M H, Stephens, P M, Bowen, G D Improving plant productivity with rhizosphere bacteria 201-203, 1994

Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize. Fems Microbiology Letters 266(2): 231-235, 2007

Perturbation of maize rhizosphere microflora following seed bacterization with Burkholderia cepacia MCI 7. FEMS Microbiology Ecology 23(3): 183-193, 1997

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy. Environmental Microbiology 3(2): 137-143, 2001

Detection of cultured and uncultured Burkholderia cepacia complex bacteria naturally occurring in the maize rhizosphere. Environmental Microbiology 7(11): 1734-1742, 2005

DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. Journal of Clinical Microbiology 38(9): 3165-3173, 2000

Investigating Burkholderia cepacia complex populations recovered from Italian maize rhizosphere by multilocus sequence typing. Environmental Microbiology 9(7): 1632-1639, 2007

Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. International Journal of Systematic and Evolutionary Microbiology 51(Pt 2): 271-279, 2001