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CDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea

CDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea

Bioscience Biotechnology and Biochemistry 63(8): 1407-1413

ISSN/ISBN: 0916-8451

PMID: 27389505

DOI: 10.1271/bbb.63.1407

The conidial germ tube of the fungus Magnaporthe grisea differentiates an infection-specific structure, an appressorium, for penetration into the host plant. Formation of the appressorium is also observed on synthetic solid substrata such as polycarbonate. We found that a plant lectin, concanavalin A, specifically suppressed the appressorium formation without affecting the germling adhesion if it was applied within 2-3 hours after germination. Standing on the result, we constructed a cDNA library that represents the early stage of germ tube development and/or appressorium formation from the 2.5-hour-old germ tubes using a cDNA subtraction strategy by the combination of the biotin labeled driver method and adapter-primed PCR method. Out of 686 colonies of the library, 158 distinct clones' nucleotide sequences were partially analyzed. Some clones' expression patterns were detected by RT-PCR and from those results, our library seemed to well represent the objective developmental stage of M. grisea.

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Accession: 003058025

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