Characterization of coastal cutthroat trout (Oncorhynchus clarki clarki) microsatellites and their conservation in other salmonids
Condrey, M.J.; Bentzen, P.
Molecular Ecology 7(6): 787-789
ISSN/ISBN: 0962-1083 PMID: 9640655 Accession: 003064832
Six primer pairs were developed to amplify microsatellite loci. When 20 coastal cutthroat trout from populations from Washington State were analysed using these primers, polymorphisms were detected for 5 of these loci. The mean number of alleles per locus was 9 and the mean heterozygosity was 0.85. Optimum PCR conditions for the use of these primers were determined. The 6 primer sets were tested on 13 additional salmonid species from 4 genera (Oncorhynchus, Salmo, Salvelinus and Thymallus).