+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of rhizobia homologues of Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4



Characterization of rhizobia homologues of Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4



FEMS Microbiology Ecology 25(4): 341-348



Homologues to Sinorhizobium meliloti insertion sequences ISRim3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially sequenced some of these putative insertion sequence elements. DNA sequence comparisons indicate that S. meliloti and R. leguminosarum ISRm3-type elements are closely related whereas the ISRm4 homologues show distinct relationships. In addition, specific primers for PCR recognition of ISRm3 and ISRm4 elements in rhizobia species and S. meliloti ISRm6 were designed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003065231

Download citation: RISBibTeXText

DOI: 10.1016/s0168-6496(98)00002-6


Related references

ISRm4-1 and ISRm9, two novel insertion sequences from Sinorhizobium meliloti. Gene (amsterdam). 207(1): 93-96,., 1998

Polyphasic taxonomy of rhizobia: Emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov. International Journal of Systematic Bacteriology 44(4): 715-733, 1994

Characterization and splicing in vivo of a Sinorhizobium meliloti group II intron associated with particular insertion sequences of the IS630-Tc1/IS3 retroposon superfamily. Molecular Microbiology 28(6): 1295-1306, 1998

Identification and nucleotide sequence of Rhizobium meliloti insertion sequence ISRm3: similarity between the putative transposase encoded by ISRm3 and those encoded by Staphylococcus aureus IS256 and Thiobacillus ferrooxidans IST2. Journal of Bacteriology 173(8): 2530-2538, 1991

Analysis of Rhizobium meliloti nodulation mutant WL131: novel insertion sequence ISRm3 in nodG and altered nodH protein product. Journal of Bacteriology 173(10): 3060-3065, 1991

Nucleotide sequence of Rhizobium meliloti GR4 insertion sequence ISRm3 linked to the nodulation competitiveness locus nfe. Plant Molecular Biology 20(2): 307-309, 1992

Analysis of different types of competitive capacity in the alfalfa rhizobia (Sinorhizobium meliloti) Tn5 mutants. Genetika Moskva 37(11): 1507-1512, 2001

Isolation and characterization of functional insertion sequences of rhizobia. Fems Microbiology Letters 261(1): 25-31, 2006

Wastewater sludge as a substrate for growth and carrier for rhizobia: the effect of storage conditions on survival of Sinorhizobium meliloti. Bioresource Technology 83(2): 145-151, 2002

IS Rm31, a new insertion sequence of the IS 66 family in Sinorhizobium meliloti. Archives of Microbiology 180(2): 118-126, 2003

Rhizobia from Lanzarote, the Canary Islands, that nodulate Phaseolus vulgaris have characteristics in common with Sinorhizobium meliloti isolates from mainland Spain. Applied and Environmental Microbiology 75(8): 2354-2359, 2009

Cultural conditions required for the induction of an adaptive acid-tolerance response (ATR) in Sinorhizobium meliloti and the question as to whether or not the ATR helps rhizobia improve their symbiosis with alfalfa at low pH. Fems Microbiology Letters 302(2): 123-130, 2010

Cultural conditions required for the induction of an adaptive acid-tolerance response in Sinorhizobium meliloti and the question as to whether or not the ATR helps rhizobia improve their symbiosis with alfalfa at low pH. Fems Microbiology Letters2: 2, 123-130, 2010

Genetic and functional characterization of a yet-unclassified rhizobial Dtr (DNA-transfer-and-replication) region from a ubiquitous plasmid conjugal system present in Sinorhizobium meliloti, in Sinorhizobium medicae, and in other nonrhizobial Gram-negative bacteria. Plasmid 67(3): 199-210, 2012

Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti. Archives of Microbiology 187(1): 79-85, 2007