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Chromosome mutations causing ColE1 plasmid replication in Agrobacterium radiobacter


Chromosome mutations causing ColE1 plasmid replication in Agrobacterium radiobacter



Genetika Moskva 35(3): 400-404



Plasmids, derivatives of ColE1, fail to replicate in bacteria not belonging to the genus Escherichia. Chromosomal mutations of Agrobacterium radiobacter 5D-1, which "afford" replication of ColE1 plasmid in cells of this soil saprophytic bacterium belonging to the Rhizobiaceae family, were identified. These mutations were obtained as a result of plasmid pSUP2021 integration into the chromosome of Agrobacterium.

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