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Classical swine fever virus: discrimination between vaccine strains and European field viruses by restriction endonuclease cleavage of PCR amplicons



Classical swine fever virus: discrimination between vaccine strains and European field viruses by restriction endonuclease cleavage of PCR amplicons



Acta Veterinaria Scandinavica 39(3): 395-400



A method for discriminating between classical swine fever (CSF) vaccine strains and recent European field viruses was developed based on the theory that vaccine and field strains formed different genogroups. An assay was developed based on the amplification of the 5'-non coding (5'-NC) region of the CSF virus genome followed by selective restriction endonuclease cleavage of the amplified DNA fragments. 13 vaccine strains and 23 European field field viruses were tested.

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Accession: 003068458

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PMID: 9787502


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