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Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli


Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli



Acta Biochimica et Biophysica Sinica 30(5): 454-458



ISSN/ISBN: 1672-9145

A 800 bp DNA fragment was amplified by RT-PCR from adult Schistosoma japonicum mRNA. Sequence analysis revealed that this fragment contained the S. japonicum (Chinese strain) triose-phosphate isomerase gene. The gene was cloned into the expression vector pGEX-4T and subsequently expressed in Escherichia coli. The recombinant GST-fusion protein was purified by glutathione agarose affinity chromatography. Its MW was 54 kDa. The yield of expression was around 30 mg/litre E. coli culture.

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Accession: 003069877

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