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Construction and preliminary assessment of the immunogenicity of the infectious bronchitis virus S1 gene DNA vaccine plasmid


Construction and preliminary assessment of the immunogenicity of the infectious bronchitis virus S1 gene DNA vaccine plasmid



Chinese Journal of Veterinary Science 18(6): 527-530



The cDNA of the S1 gene was transferred from pUCQDS1 to pSY538 to add the end code TAA at its 3' end. After that, the fragment containing the intact open reading frame of the S1 gene was inserted into pSV- beta -LacZ down-stream of the SV40 enhancer and promoter and up-stream of the SV40 poly A signal to replace the LacZ gene.

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